Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
5:31
Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
6:31
Conclusion
필기록
The overall goal of this protocol is to visualize step-wise procedures involved in the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with chemical and physical agents of interest. Thi
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
We describe a detailed protocol for the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with a chemical/physical agent of interest. This approach has been widely used for mutagenicity testing of carcinogens in mammalian cells.