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A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

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09:04 min

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November 7th, 2019

DOI :

10.3791/60486-v

November 7th, 2019

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9,124 Views

Carmen G. Flesher¹, Nicki A. Baker¹, Clarissa Strieder-Barboza¹^,², Dominic Polsinelli⁵, Phillip J. Webster¹^,², Oliver A. Varban¹, Carey N. Lumeng²^,³^,⁴, Robert W. O'Rourke¹^,⁶

¹Department of Surgery, University of Michigan Medical School, ²Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, ³Graduate Program in Immunology, University of Michigan Medical School, ⁴Graduate Program in Cellular and Molecular Biology, University of Michigan Medical School, ⁵Undergraduate Research Opportunity Program, University of Michigan, ⁶Department of Surgery, Ann Arbor Veterans Affairs Healthcare System

章

文字起こし

要約

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3D Extracellular Matrix

Adipocyte Culture

Metabolic Crosstalk

Adipogenic Differentiation

Type 2 Diabetes

Visceral Adipose Tissue

Preadipocyte Isolation

Decellularization

Collagenase Digestion

ECM Preparation

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