Adipose Tissue Procurement and Preadipocyte Isolation
2:51
Adipose Tissue ECM Preparation
5:08
ECM-Adipocyte Preparation
7:04
Results: Genotypic and Phenotypic Characterization of In vitro Differentiated Adipocytes
8:36
Conclusion
Transcript
Our protocol demonstrates that the ECM may promote adipogenic differentiation in the absence of classic adipogenic mediators and is the first to demonstrate disease-specific regulation of cellular metabolism by the ECM. Our technique provides a to
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We describe a 3D human extracellular matrix-adipocyte in vitro culture system that permits dissection of the roles of the matrix and adipocytes in contributing to adipose tissue metabolic phenotype.