This work was financially supported by the National Natural Science Foundation of China (82204088, 82273669) and the Natural Science Foundation of Shandong Province, China (ZR2021QH209).

We used male C57BL/6J mice (age: 6-8 weeks; weight: 20-22 g) for all behavioral tests. The mice were subjected to steady conditions (i.e., temperature, 23 &#177; 1 &#176;C; humidity, 55% &#177; 5%, and 12/12 h light-dark cycle with lights on at 7:00). All behavioral tests were performed between 10:00 and 17:00. All animal experiments were approved by the Ethics Committee of the Professional Committee of Animal Experiments of Qingdao University. After a 1-week acclimation period, all mice were exposed to inhalable environ...

Assessing Olfactory Impairment in Mice Due to Inhaled Environmental Pollutants

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The authors declare no conflicts of interest.

This article introduces two fundamental protocols designed for the swift assessment of olfactory impairment in mice. Varied inhalable environmental pollutants result in distinct levels of olfactory dysfunction in mice. The buried food test is employed to assess the capability to detect volatile odors, while the social odor discrimination experiment evaluates the animal&#39;s capacity to discern and differentiate various social odors. The protocol here serves to evaluate the toxic effects of environmental pollutants on ol...

Olfactory impairment has emerged as a noteworthy public health concern and is independently associated with an increased risk of neurodegenerative diseases. This condition can adversely affect overall well-being, contribute to the development of depressive symptoms, and result in a diminished quality of life. Its impact is prominently observed in the altered perception of food, hindrance in social communication, and heightened negative feelings1. Various factors, including sinonasal disease, upper respiratory tract infection, and traumatic brain injury, have been considered contributors to olfactory impairment in humans2. Notably, inhalable environmental pollutants such as PM2.5, estimated to range from 2% to 16%, enter the body through inspired air, traverse the nasal cavity, and reach specific olfaction-dedicated regions where they are deposited3,4,5,6,7. Recent findings indicate that inhalable environmental pollutants, including PM2.5 and ammonia, can indeed harm olfactory sensory neurons8,9,10. However, further validation is required to ascertain whether such damage directly leads to olfactory dysfunction. Hence, a meticulous evaluation of the effects of inhalable environmental pollutants on olfactory function is of particular importance.
Currently, numerous research laboratories employ mice as an alternative vertebrate model for behavioral experiments aimed at comprehending changes in olfactory function11,12,13,14. Mice were chosen as the preferred model system for investigating vertebrate chemical communication, and it exhibits a remarkable olfactory sensitivity crucial for foraging and social communication15. Furthermore, the continually evolving array of tools for observing and influencing mouse behavior has rendered this species exceptionally appealing for research on olfactory function16.
In this study, we employed the buried food test and the social odor discrimination experiment to assess olfactory impairment in a mouse model exposed to inhalable environmental pollutants. To enhance the precision of evaluation, we opted for the most representative method for assessing olfactory function. We systematically refined this method to ensure simplicity and clarity, allowing us to gauge the extent of olfactory dysfunction induced by inhalable environmental pollutants effectively.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0.5-10 &#956;L&#160; adjustable micropipette</td>
			<td>Eppendorf, Germany</td>
			<td>3123000225</td>
			<td>Intranasal instillation</td>
		</tr>
		<tr>
			<td>0.9% saline solution</td>
			<td>Solarbio</td>
			<td>7647-14-5</td>
			<td>Dissolve pollutants</td>
		</tr>
		<tr>
			<td>Anhydrous zinc sulfate</td>
			<td>Macklin</td>
			<td>7733-02-0</td>
			<td>Expose mice</td>
		</tr>
		<tr>
			<td>Centrifuge tube (2 mL)</td>
			<td>Biosharp Incorporated</td>
			<td>BS-20-M</td>
			<td>Place urine</td>
		</tr>
		<tr>
			<td>Electronic balance</td>
			<td>Changzhou Ohaus Co.</td>
			<td>EX125DZH</td>
			<td>Weight anesthetics and pollutants</td>
		</tr>
		<tr>
			<td>GraphPad Prism</td>
			<td>GraphPad Software</td>
			<td>8.0.1</td>
			<td>statistic analysis</td>
		</tr>
		<tr>
			<td>Handheld Dust detector</td>
			<td>TSI&#160;Incorporated</td>
			<td>DuatTrak <img alt="Equation 1" src="/files/ftp_upload/66818/66818eq01.jpg" style="margin: auto;" />8532</td>
			<td>Inhalation-exposed mice</td>
		</tr>
		<tr>
			<td>Video recording equipment</td>
			<td>Apple Inc.</td>
			<td>iPhone 6s Plus</td>
			<td>The activity time of mice was recorded</td>
		</tr>
		<tr>
			<td>Vortex mixer</td>
			<td>Haimen Kylin-Bell Lab Instruments Co.</td>
			<td>Vortex-5&#160;</td>
			<td>Mix solution</td>
		</tr>
	</tbody>
</table>

studying the effects of inhaled environmental pollutants on olfactory function in mice

Olfactory impairment is a significant public health problem and independently predicts the risk of neurodegenerative diseases. Inhaled environmental pollutants exposure may impair olfaction; thereby, there is an urgent need for methods to evaluate the effects of inhaled environmental pollutants exposure on olfaction. Mice are ideal models for olfactory experiments because of their highly developed olfactory system and behavioral characteristics. To assess the effects of inhaled environmental pollutants exposure on olfactory function in mice, a detailed buried food test and social odor discrimination experiment is provided, including the experiment preparation, the selection and construction of experimental facilities, the testing process, and indexes of time. Meanwhile, timekeeping equipment, operational details, and the experimental environment are discussed to ensure the success of the assay. Zinc sulfate is used as the treatment to demonstrate the feasibility of the experimental approach. The protocol provides a simple and clear operational process for assessing the effects of inhaled environmental pollutants on olfactory function in mice.

Author Spotlight: Assessing the Olfactory Effects of Airborne Pollutants &#8212; Buried Food and Social Odor Tests

Studying the Effects of Inhaled Environmental Pollutants on Olfactory Function in Mice

Our research is environmental toxicology. It asks to provide two behavioral testing methods for starting effects or inheritable environmental pollutants on olfactory function. We provide two simple and effective methods by certain food tests and the social order discrimination experiment for determining the toxicity of inheritable environmental pollutants on olfactory function.We can comprehensively evaluate the impact of inheritable environmental pollutants on olfactory function by convincing the variable test and social order discrimination experiment matters. Our protocol uses a video device to time and record mouse behavior, addressing the efficiency of observes not being able to observe the behavior of mice in the observation area.

Inhalable environmental pollutants impair olfactory function in mice. Atmospheric zinc emitted from incinerators and motor vehicles has been demonstrated to be an inhaled pollutant that can result in allergic lung inflammation20. Zinc sulfate is considered one of the typical compounds to cause olfactory dysfunction21. Therefore, we use zinc sulfate as the treatment to expose mice by intranasal instillation and test using the buried food test and social odor discrimination e...

This paper provides a detailed description of the buried food test and social odor discrimination experiment to assess the effects of inhaled environmental pollutants exposure on olfactory function in mice.

Olfactory impairment is a significant public health problem and independently predicts the risk of neurodegenerative diseases. Inhaled environmental ...

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JoVE Journal

Environment

198 ビュー.  Qingdao University. 私たちの研究は環境毒性学です。それは、嗅覚機能に対する開始効果または遺伝性環境汚染物質のための2つの行動試験方法を提供することを求めています。私たちは、遺伝性環境汚染物質の嗅覚機能に対する毒性を決定するための、特定の食品試験と社会秩序識別実験による2つの簡単で効果的な方法を提供します。遺伝性環境汚染物質が?...