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Karlsruhe Institute of Technology (KIT)

7 ARTICLES PUBLISHED IN JoVE

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Chemistry

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes
Peter Lindemann 1, Yannick Träutlein 1, Christof Wöll 1, Manuel Tsotsalas 1
1Institute of Functional Interfaces, Karlsruhe Institute of Technology

In this paper we describe the interfacial synthesis of conjugated microporous polymers (CMP) on sacrificial substrates, and the dissolution of the substrate for the preparation of freestanding CMP nanomembranes. In addition, we will describe how the fragile nanomembranes can be transferred to other substrates.

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Neuroscience

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
Satoru Yamagishi 1, Gandhervin Kesavamoorthy 1, Martin Bastmeyer 2, Kohji Sato 1
1Anatomy and Neuroscience, Hamamatsu University School of Medicine, 2Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT)

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.

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Biology

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
Johannes F. Buyel 1,2, Jürgen Hubbuch 3, Rainer Fischer 1,2
1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology, RWTH Aachen University, 3Department of Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT)

Three heat precipitation methods are presented that effectively remove more than 90% of host cell proteins (HCPs) from tobacco extracts prior to any other purification step. The plant HCPs irreversibly aggregate at temperatures above 60 °C.

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Bioengineering

Fast Pyrolysis of Biomass Residues in a Twin-screw Mixing Reactor
Axel Funke 1, Daniel Richter 1, Andreas Niebel 1, Nicolaus Dahmen 1, Jörg Sauer 1
1Institute of Catalysis Research and Technology (IKFT), Karlsruhe Institute of Technology (KIT)

A procedure for thermochemical conversion of biomass residues is presented that aims at maximizing the yield of liquid products (fast pyrolysis). It is based on a technology proven on an industrial scale and especially suitable for treating a straw type of biomass.

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Engineering

Adsorption Device Based on a Langatate Crystal Microbalance for High Temperature High Pressure Gas Adsorption in Zeolite H-ZSM-5
Wenjin Ding 1, Giulia Baracchini 1, Michael Klumpp 2, Wilhelm Schwieger 2, Roland Dittmeyer 1
1Institute for Micro Process Engineering, Karlsruhe Institute of Technology (KIT), 2Institute of Chemical Reaction Engineering, University Erlangen-Nürnberg (FAU)

A protocol for high-temperature and high-pressure gas adsorption measurements on zeolite H-ZSM-5 using an adsorption measurement device based on a langatate crystal microbalance is presented. Prior to the adsorption measurements, the synthesis of zeolite H-ZSM-5 on the langatate crystal microbalance sensor by the steam-assisted crystallization (SAC) method is demonstrated.

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Developmental Biology

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Irene U. Wacker 1,2, Lisa Veith 3, Waldemar Spomer 2,4, Andreas Hofmann 2,4, Marlene Thaler 5, Stefan Hillmer 6, Ulrich Gengenbach 2,4, Rasmus R. Schröder 1,2,3
1Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility, Universität Heidelberg

This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.

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JoVE Journal

Synthesis of Bimetallic Pt/Sn-based Nanoparticles in Ionic Liquids
Christine Dietrich 1,2, Georgios Uzunidis 1,2, Yannick Träutlein 1, Silke Behrens 1,2
1Karlsruhe Institute of Technology (KIT), 2Ruprecht-Karls University of Heidelberg

A protocol for the synthesis of bimetallic nanoparticles in ionic liquids and the procedure of their catalytic testing in the selective hydrogenation of unsaturated aldehydes are described.

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