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Dana Farber Cancer Institute

6 ARTICLES PUBLISHED IN JoVE

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Biology

Olfactory Behavioral Testing in the Adult Mouse
Rochelle M. Witt 1,2, Meghan M. Galligan 1,2, Jennifer R. Despinoy 1,2, Rosalind Segal 1,2
1Department of Pediatric Oncology, Dana Farber Cancer Institute, 2Department of Neurobiology, Harvard Medical School

Fundamental, yet unique properties of the rodent olfactory system have led to its increasing study among biologists. A relatively simple assessment of its function is then also needed. Here we describe sensitive tests for the characterization of mouse olfactory sensitivity and preference.

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Biology

Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
Maria F. Pazyra-Murphy 1,2, Rosalind A. Segal 1,2
1Department of Pediatric Oncology, Dana Farber Cancer Institute, 2Department of Neurobiology, Harvard Medical School

Here we describe the technique of preparing and maintaining compartmented chambers for culturing sensory neurons of the dorsal root ganglia.

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Biology

Retro-orbital Injection in Adult Zebrafish
Emily K. Pugach 1,2, Pulin Li 1,2, Richard White 1,2,3, Leonard Zon 1,2
1Department of Hematology and Oncology, Children’s Hospital Boston, 2Harvard Stem Cell Institute, Harvard Medical School, 3Department of Medical Oncology, Dana Farber Cancer Institute

Here we show how to do retro-orbital injection in adult zebrafish.

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Immunology and Infection

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
Jeff K. Davies 1,2, Christine M. Barbon 1, Annie R. Voskertchian 1, Lee M. Nadler 1,2, Eva C. Guinan 3,4
1Medical Oncology, Dana Farber Cancer Institute, 2Department of Medicine, Brigham and Womens Hospital, 3Pediatric Oncology, Dana Farber Cancer Institute, 4Division of Hematology/Oncology, Children’s Hospital Boston

This paper describes a simple technique to induce alloantigen-specific anergy in human peripheral blood mononuclear cells. The technique can be applied clinically to generate non-alloreactive donor cells. Infusion of these cells could improve immune reconstitution and reduce toxicity after allogeneic hematopoietic stem cell transplantation.

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Immunology and Infection

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Mandy Muller *1,2, Patricia Cassonnet *1, Michel Favre 1, Yves Jacob 1,3, Caroline Demeret 1
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur , 2Cellule Pasteur, Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute

This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.

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Medicine

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
Emily J. Chadwick 1, David P. Yang 1, Mariella G. Filbin 2, Emanuele Mazzola 3, Yu Sun 1, Oded Behar 1,4, Maria F. Pazyra-Murphy 1, Liliana Goumnerova 5, Keith L. Ligon 6, Charles D. Stiles 1, Rosalind A. Segal 1
1Department of Cancer Biology, Dana-Farber Cancer Institute, 2Department of Pediatrics, Children's Hospital, 3Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, 5Department of Neurosurgery, Children's Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute

Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.  

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