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Geneplus-Beijing Institute

4 ARTICLES PUBLISHED IN JoVE

Cancer Research

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Xiaoxing Lv¹, Meiru Zhao¹, Yuting Yi¹, Lucheng Zhang¹, Yanfang Guan¹, Tao Liu¹, Ling Yang¹, Rongrong Chen¹, Jianhui Ma¹, Xin Yi¹

¹Geneplus-Beijing Institute

This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

Cancer Research

Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

Jorge A. Benitez^*1, Ciro Zanca^*1, Jianhui Ma¹, Webster K. Cavenee^1,2,3, Frank B. Furnari^1,2,4

¹Ludwig Institute for Cancer Research, ²Moores Cancer Center, School of Medicine, University of California, San Diego, ³Department of Pathology, School of Medicine, University of California, San Diego, ⁴Department of Medicine, School of Medicine, University of California, San Diego

Orthotopic intracranial injection of tumor cells has been used in cancer research to study brain tumor biology, progression, evolution, and therapeutic response. Here we present fluorescence molecular tomography of tumor xenografts, which provides real-time intravital imaging and quantification of a tumor mass in preclinical glioblastoma models.

Neuroscience

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Mengye Zhu¹, Daying Zhang¹, Sicong Peng², Nana Liu², Jing Wu², Haixia Kuang², Tao Liu^2,3

¹Department of Pain, First Affiliated Hospital of Nanchang University, ²Department of Pediatrics, First Affiliated Hospital of Nanchang University, ³Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

Medicine

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

Tao Liu^*1,2,3,4,5, Xueling Song^*1,2,3,4,5, Xiaoying Zheng^*1,2,3,4,5, Liying Yan^1,2,3,4,5, Caihong Ma^1,2,3,5, Rong Li^1,2,3,4,5, Jie Yan^1,2,3,4,5, Jie Qiao^1,2,3,4,5

¹Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, ²National Clinical Research Center for Obstetrics and Gynecology, ³Key Laboratory of Assisted Reproduction, Ministry of Education, ⁴Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, ⁵Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences

In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation.