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University of Hawai'i at Mānoa

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Shruti Shankar^1,2, Meredith E.K. Calvert^3,4, Joanne Y. Yew^1,2,5

¹Temasek Life Sciences Laboratory, ²Department of Biological Science, National University of Singapore, ³Bioimaging and Biocomputing Facility, Temasek Life Sciences Laboratory, ⁴Histology and Light Microscopy Core, Gladstone Institutes, ⁵Pacific Biosciences Research Center, University of Hawai'i at Mānoa

The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.

Chemistry

High Pressure Single Crystal Diffraction at PX^2

Dongzhou Zhang¹, Przemyslaw K. Dera¹, Peter J. Eng², Joanne E. Stubbs², Jin S. Zhang¹, Vitali B. Prakapenka², Mark L. Rivers²

¹Hawai’i Institute of Geophysics and Planetology, University of Hawai’i at Manoa, ²Center for Advanced Radiation Sources, University of Chicago

In this report, we describe detailed procedures for carrying out single crystal X-ray diffraction experiments with a diamond anvil cell at the GSECARS 13-BM-C beamline at the Advanced Photon Source. ATREX and RSV programs are used to analyze the data.

Behavior

Behavioral Tracking and Neuromast Imaging of Mexican Cavefish

McLean Worsham¹, Vânia Filipa Lima Fernandes², Alexander Settle¹, Chantell Balaan², Kimberly Lactaoen², Lillian J. Tuttle^1,3, Motoko Iwashita¹, Masato Yoshizawa¹

¹Department of Biology, University of Hawai'i at Mānoa, ²Department of Anatomy, Biochemistry and Physiology, University of Hawai'i at Mānoa, ³Pacific Biosciences Research Center, University of Hawai'i at Mānoa

Here, we present methods for high-throughput study of a series of the Mexican cavefish behaviors and vital staining of a mechanosensory system. These methods use free-software and custom-made scripts, providing a practical and cost-effective method for the studies of behaviors.

Chemistry

An Externally-Heated Diamond Anvil Cell for Synthesis and Single-Crystal Elasticity Determination of Ice-VII at High Pressure-Temperature Conditions

Xiaojing Lai^1,2, Feng Zhu², Jin S. Zhang³, Dongzhou Zhang^2,4, Sergey Tkachev⁴, Vitali B. Prakapenka⁴, Bin Chen²

¹Gemmological Institute, China University of Geosciences (Wuhan), ²Hawai'i Institute of Geophysics and Planetology, University of Hawai'i at Mānoa, ³Department of Earth and Planetary Sciences, University of New Mexico, ⁴Center for Advanced Radiation Sources, University of Chicago

This work focuses on the standard protocol for preparing the externally-heated diamond anvil cell (EHDAC) for generating high-pressure and high-temperature (HPHT) conditions. The EHDAC is employed to investigate materials in Earth and planetary interiors under extreme conditions, which can be also used in solid state physics and chemistry studies.

JoVE Core

Determining the Mechanical Strength of Ultra-Fine-Grained Metals

Jianing Xu^*1,2, Yanju Wang^*2, Jinyuan Yan³, Bin Chen^1,2

¹School of Science, Harbin Institute of Technology, ²Center for High Pressure Science and Technology Advanced Research, ³Advanced Light Source, Lawrence Berkeley National Lab

The protocol presented here describes the high-pressure radial diamond-anvil-cell experiments and analyzing the related data, which are essential for obtaining the mechanical strength of the nanomaterials with a significant breakthrough to the traditional approach.