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Xiangya Second Hospital of Central South University, Changsha, China

5 ARTICLES PUBLISHED IN JoVE

Medicine

Technical Aspects of the Mouse Aortocaval Fistula

Kota Yamamoto^1,2, Xin Li^1,3, Chang Shu³, Tetsuro Miyata², Alan Dardik^1,4

¹The Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, ²Department of Vascular Surgery, The University of Tokyo, ³Department of Vascular Surgery, Central South University, ⁴VA Connecticut Healthcare Systems

The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.

Medicine

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Hualong Bai^1,2,3,4,5, Xin Li⁶, Takuya Hashimoto^1,2,5, Haidi Hu^1,2,5, Trenton R. Foster^1,2,5, Jesse J. Hanisch^1,2,5, Jeans M. Santana^1,2,5, Alan Dardik^1,2,5

¹Department of Surgery, Yale University, ²Vascular Biology and Therapeutics Program, Yale University, ³Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, ⁴Basic Medical College of Zhengzhou University, ⁵VA Connecticut Healthcare Systems, West Haven, CT, ⁶Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China

We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation.

Engineering

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Tianzhuo Zhao^1,2,3, Xin Li^1,2, Qixiu Zhong^1,2, Hong Xiao^1,3, Shuzhen Nie^1,3, Fuqiang Lian^1,3, Sining Sun⁴, Zhongwei Fan^1,2,3

¹The Academy of Opto-electronics, Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³National Engineering Research Center for DPSSL, ⁴Beijing GK Laser Technology Co., Ltd.

During vacuum induction melting, laser-induced breakdown spectroscopy is used to perform real-time quantitative analysis of the main-ingredient elements of a molten alloy.

Developmental Biology

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Mengrou Liu^*1, Chunsen Zhu^*1, Shun Bai^*2, Xin Li¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Immunology and Infection

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Xin Li¹, Zhengmei Mao², Li Yang¹, Kai Sun¹

¹Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston, ²Microscopy Core, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston

New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.