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Colorado State University-Pueblo

6 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Sang-Hyuck Park 1, Rebecca Garlock Ong 2, Chuansheng Mei 3, Mariam Sticklen 4
1The School of Plant Sciences, University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, 3The Institute for Sustainable and Renewable Resources, The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences, Michigan State University

A double stranded RNA interference (dsRNAi) technique is employed to down-regulate the maize cinnamoyl coenzyme A reductase (ZmCCR1) gene to lower plant lignin content. Lignin down-regulation from the cell wall is visualized by microscopic analyses and quantified by the Klason method. Compositional changes in hemicellulose and crystalline cellulose are analyzed.

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Biology

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
Elizabeth K. Peterson 1,2, Hugh E. Long 1,2
1Communities for Building Active STEM Engagement, Colorado State University-Pueblo, 2Department of Biology, Colorado State University-Pueblo

In this paper, we provide a detailed protocol for exposing species in the genus Drosophila to pollutants with the goal of studying the impact of exposure on a range of phenotypic outputs at different developmental stages and for more than one generation.

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Biochemistry

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass
Kenneth J. Olejar *1, Min Hong *2, Sun-Yeop Lee 2, Tae-Hyung Kwon 2, Soo-Ung Lee 2, Chad A. Kinney 1,3, Joon-Hee Han 2, Sang-Hyuck Park 3
1Department of Chemistry, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute for Cannabis Research, Colorado State University-Pueblo

Ultrasonic-assisted extraction (UAE) increases extraction efficiency of solvents and when applied to Cannabis spp. biomass it reduces the time required for extraction. This decreases the cost and potential cannabinoid loss due to degradation. Additionally, UAE is considered a green method due to low solvent use.

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Bioengineering

Employing Aeroponic Systems for the Clonal Propagation of Cannabis
Trevor Regas 1, Joon-Hee Han 2, Christopher S. Pauli 3, Sang-Hyuck Park 1,3
1Biology Department, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute of Cannabis Research, Colorado State University-Pueblo

This protocol is designed to provide instructional information for the clonal propagation of Cannabis sativa L. by implementing aeroponic systems. The method described here includes all necessary supplies and protocols to successfully reproduce desirable morphological and chemical properties in the genus Cannabis.

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Biology

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies
Sang-Hyuck Park 1, Samuel Koch 2, Katherine Richardson 3, Christopher Pauli 1, Joon-Hee Han 4, Tae-Hyung Kwon 4
1Institute of Cannabis Research, Colorado State University-Pueblo, 2Department of Biology, Colorado State University-Pueblo, 3Department of Chemistry, Colorado State University-Pueblo, 4Chuncheon Bioindustry Foundation

The present protocol provides instructional information for using tobacco hornworm Manduca sexta in cannabinoid research. The method described here includes all necessary supplies and protocols to monitor physiological and behavioral changes of the insect model in response to cannabidiol (CBD) treatment.

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Biology

Mouse Lumbar Vertebra Uniaxial Compression Testing with Embedding of the Loading Surface
Chandler A. Sparks 1,2, Ryeim B. Ansaf 1,3, Annette M. Gabaldón 1
1Department of Biology, Colorado State University-Pueblo, 2Hackensack Meridian School of Medicine, 3Department of Engineering, Colorado State University-Pueblo

In this protocol, two approaches are described to make uniaxial compression testing of mouse lumbar vertebrae more attainable. First, the conversion of a three-point bending machine to a compression testing machine is described. Second, an embedding method for preparing the loading surface that uses bone cement is adapted for mouse lumbar vertebrae.

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