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Indiana University Melvin and Bren Simon Cancer Center

4 ARTICLES PUBLISHED IN JoVE

Medicine

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of β-aminopropionitrile-induced Aortic Aneurysm and Dissection

Brittany O. Aicher¹, Subhradip Mukhopadhyay¹, Xin Lu², Selen C. Muratoglu¹, Dudley K. Strickland¹, Areck A. Ucuzian^1,3

¹Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, ²Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, ³Division of Vascular Surgery, University of Maryland School of Medicine

This article describes a detailed methodology of using a radiopaque lead-based silicone rubber to perfuse the murine vasculature for aortic diameter quantification in a mouse model of aortic aneurysm and dissection.

Biology

A Proximal Culture Method to Study Paracrine Signaling Between Cells

Subramanyam Dasari¹, Taruni Pandhiri¹, James Haley¹, Dean Lenz², Anirban K. Mitra^1,3,4

¹Indiana University School of Medicine, ²Urology, Indiana University Health Southern Indiana Physicians, ³Indiana University Melvin and Bren Simon Cancer Center, ⁴Department of Medical and Molecular Genetics, Indiana University School of Medicine

Paracrine and juxtacrine cellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Here, a proximal culture method is used to study paracrine signaling where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.

Cancer Research

An Ivor Lewis Esophagectomy Designed to Minimize Anastomotic Complications and Optimize Conduit Function

Neal K. Ramchandani¹, Kenneth A. Kesler¹, Jonathon D. Rogers¹, Nakul Valsangkar¹, Samatha M. Stokes¹, Shadia I. Jalal²

¹Department of Surgery, Division of Cardiothoracic, Indiana University Melvin and Bren Simon Cancer Center, ²Department of Medicine, Medical Oncology Division, Indiana University Melvin and Bren Simon Cancer Center

We describe a novel intrathoracic esophagogastric anastomotic technique designed to create a large diameter anastomosis while simultaneously maintaining conduit blood supply to minimize the incidence of anastomotic leaks and strictures. Construction and orientation of the stomach conduit designed to optimize upper gastrointestinal tract function is also described.

Biochemistry

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Shan Feng^1,2, Xiaofei Wen³, Xin Lu^2,4,5,6

¹Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, ²Department of Biological Sciences, University of Notre Dame, ³Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, ⁴Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, ⁵Harper Cancer Research Institute, University of Notre Dame, ⁶Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

Proteomic profiling of tyrosine-nitrated proteins has been a challenging technique due to the low abundance of the 3-nitrotyrosine modification. Here we describe a novel approach for nitropeptide enrichment and profiling by using Angiotensin II as the model. This method can be extended for other in vitro or in vivo systems.