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University of Munich

4 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
Dirk Steinritz 1,2, Annette Schmidt 1,3, Frank Balszuweit 1, Horst Thiermann 1, Marwa Ibrahim 3, Birgit Bölck 3, Wilhelm Bloch 3
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, 3Department of Molecular and Cellular Sports Medicine, German Sports University Cologne

Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.

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Genetics

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
Christopher T. Breunig 1,2, Andrea M. Neuner 1,2, Jessica Giehrl-Schwab 3, Wolfgang Wurst 3, Magdalena Götz 2,4, Stefan H. Stricker 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

Here, we present string assembly gRNA cloning (STAgR), a method to easily multiplex gRNA vectors for CRISPR/Cas9 approaches. STAgR makes gRNA multiplexing simple, efficient and customizable.

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Medicine

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
Amelie Tsoutsoulopoulos 1, Katrin Gohlsch 2, Niklas Möhle 3, Andreas Breit 2, Sebastian Hoffmann 5, Olaf Krischenowski 3,4, Harald Mückter 2, Thomas Gudermann 2, Horst Thiermann 1, Michaela Aufderheide 3,4, Dirk Steinritz 1,2
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, University of Munich, 3Cultex Laboratories GmbH, 4Cultex Technologies GmbH (formerly Cultex Laboratories GmbH), 5seh consulting + services

We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).

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Neuroscience

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
Christian Friess 1, Magdalena Götz 1,2,3, Jacob Kjell 1,2,4
1Division of Physiological Genomics, Biomedical Center, Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research, Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology, University of Munich, 4Department of Clinical Neuroscience, Karolinska Institutet

Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

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