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Nationwide Children’s Hospital

6 ARTICLES PUBLISHED IN JoVE

Medicine

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Yong-Ung Lee¹, Tai Yi¹, Shuhei Tara¹, Avione Y. Lee¹, Narutoshi Hibino¹, Toshiharu Shinoka², Christopher K. Breuer^1,3

¹Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, ²Department of Cardiothoracic Surgery, Nationwide Children's Hospital, ³Pediatric Surgery, Nationwide Children's Hospital

To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.

Medicine

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Yong-Ung Lee¹, Tai Yi¹, Iyore James¹, Shuhei Tara¹, Alexander J. Stuber¹, Kejal V. Shah¹, Avione Y. Lee¹, Tadahisa Sugiura¹, Narutoshi Hibino², Toshiharu Shinoka¹, Christopher K. Breuer^1,3

¹Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, ²Cardiothoracic Surgery, Nationwide Children's Hospital, ³Pediatric Surgery, Nationwide Children's Hospital

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

Genetics

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Camila F. Almeida¹, Emma C. Frair¹, Nianyuan Huang¹, Reid Neinast², Kim L. McBride^2,3,4,6, Robert B. Weiss⁵, Kevin M. Flanigan^1,6, Nicolas Wein^1,6

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, ³The Heart Center, Nationwide Children’s Hospital, ⁴Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, ⁵Department of Human Genetics, The University of Utah School of Medicine, ⁶Department of Pediatrics, The Ohio State University

This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.

Developmental Biology

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Shiqiao Ye^1,2, Xiaoping Wan³, Juan Su^1,2, Akshar Patel^1,2,4, Blake Justis^1,2,5, Isabelle Deschênes³, Ming-Tao Zhao^1,2,5,6

¹Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, ²The Heart Center, Nationwide Children’s Hospital, ³Department of Physiology and Cell Biology, The Ohio State University College of Medicine, ⁴Department of Anatomy, The Ohio State University College of Medicine, ⁵MCDB Graduate Program, The Ohio State University, ⁶Department of Pediatrics, The Ohio State University College of Medicine

Here, we present a protocol to robustly generate and expand human cardiomyocytes from patient peripheral blood mononuclear cells.

Engineering

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Yifei Liu¹, Yong-Ung Lee², Tai Yi², Ken Wu³, Cedric Bouchet-Marquis³, Han Chan³, Christopher K. Breuer², David W. McComb¹

¹Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, ²Center for Regenerative Medicine, Nationwide Children’s Hospital, ³Thermo Fisher Scientific

Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

Medicine

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography

Qiang Zeng¹, Doug A. Gouchoe^2,3, Mahboubeh Nabavinia⁴, Yong Gyu Lee², Xi Wang², Terri A. Shaffer⁵, Mitchel R. Stacy^4,5,6, Blake R. Peterson^7,8, Bryan A. Whitson^2,9, Christopher Breuer^1,4, Sylvester M. Black^1,10

¹Department of Surgery, Nationwide Children’s Hospital, ²COPPER Laboratory, The Ohio State University, ³88th Surgical Operation Operations Squadron, WPAFB, ⁴Center for Regenerative Medicine, The Research Institute at Nationwide Children’s Hospital, ⁵Division of Vascular Diseases and Surgery, The Ohio State University College of Medicine, ⁶Interdisciplinary Biophysics Graduate Program, The Ohio State University, ⁷Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University College of Pharmacy, ⁸The Ohio State University Comprehensive Cancer Center, ⁹Division of Cardiac Surgery, Department of Surgery, The Ohio State Wexner Medical Center, ¹⁰Division of Transplantation, Department of Surgery, The Ohio State Wexner Medical Center

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.