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The Affiliated Drum Tower Hospital of Nanjing University Medical School

7 ARTICLES PUBLISHED IN JoVE

Biology

A Protocol for Computer-Based Protein Structure and Function Prediction

Ambrish Roy^1,2, Dong Xu¹, Jonathan Poisson¹, Yang Zhang^1,2

¹Center for Computational Medicine and Bioinformatics, University of Michigan , ²Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

Chemistry

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering

Youngkyu Han¹, Suk-kyun Ahn^2,3, Zhe Zhang^1,4, Gregory S. Smith¹, Changwoo Do¹

¹Biology and Soft Matter Division, Neutron Science Directorate, Oak Ridge National Laboratory, ²Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, ³Department of Polymer Science and Engineering, Pusan National University, ⁴Jülich Center for Neutron Science, Forschungszentrum Jülich

A method for the functionalization of carbon nanotubes with structure-tunable polymeric encapsulation layers and structural characterization using small-angle neutron scattering is presented.

JoVE Core

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Yang Zhang^*1, Lifeng Shen^*2, Ping'er Wang³, Weixing Xi⁴, Zhongming Yu¹, Xiaowen Huang¹, Xuping Wang¹, Dan Shou¹

¹Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, ²Department of Orthopaedic Surgery, Tongde Hospital of Zhejiang Province, ³Institute of Orthopaedics and Traumatology, First Affiliated Hospital of Zhejiang Chinese Medical University, ⁴Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province

This study presents an improved rabbit model infected with Staphylococcus aureus by blocking the same amount of bacteria in bone marrow. Vancomycin loaded calcium sulphate and autogenous bone are used for antibiotic and bone repair treatment. The protocol could be helpful for studying bone infection and regeneration.

Engineering

A Soft Tooling Process Chain for Injection Molding of a 3D Component with Micro Pillars

Yang Zhang¹, David Bue Pedersen¹, Michael Mischkot¹, Matteo Calaon¹, Federico Baruffi¹, Guido Tosello¹

¹Department of Mechanical Engineering, Technical University of Denmark

A protocol for fabricating injection molding inserts for complex geometry with micro features on surfaces employing Additive Manufacturing (AM) is presented.

Neuroscience

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

Nannan Zhang¹, Zhe Zhang^1,2, Ilker Ozden², Shinghua Ding^1,2

¹Dalton Cardiovascular Research Center, University of Missouri-Columbia, ²Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

This proctocol aims to provide a method for in vitro and in vivo mitochondrial Ca²⁺ imaging in astrocytes and neurons.

Medicine

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Chenhao Hu^*1,2,3, Zhe Zhang^*1,2,3, Lei Zhang^1,2,3, Ruihan Liu^2,3, Jun Yan^1,2,3, Qi Sun^1,2,3, Guanghui Wang¹, Junjun She^1,2,3

¹Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, ²Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, ³Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

Developmental Biology

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang^*1,2,3, Zhe Zhang^*1,2,3, Nannan Kang^1,2,3, Xiaoqiang Sheng^1,2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ³Center for Molecular Reproductive Medicine, Nanjing University

Here, we describe the method of generating an artificial decidualization model using the ovariectomized mouse, a classic endometrial decidualization experiment in the research field of endometrial decidualization.