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University of Idaho

4 ARTICLES PUBLISHED IN JoVE

Environment

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Joseph B. DeShields¹, Rachel A. Bomberger¹, James W. Woodhall², David L. Wheeler¹, Natalia Moroz¹, Dennis A. Johnson¹, Kiwamu Tanaka¹

¹Department of Plant Pathology, Washington State University, ²Parma Research and Extension Center, University of Idaho

Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.

Biochemistry

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Joanna Kud¹, Wenjie Wang², Yulin Yuan², Allan Caplan², Joseph C. Kuhl², Louise-Marie Dandurand¹, Fangming Xiao²

¹Department of Entomology, Plant Pathology and Nematology, University of Idaho, ²Department of Plant Sciences, University of Idaho

The goal of this manuscript is to present an outline for the comprehensive biochemical and functional studies of the RING-type E3 ubiquitin ligases. This multistep pipeline, with detailed protocols, validates an enzymatic activity of the tested protein and demonstrates how to link the activity to function.

Immunology and Infection

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Travis J. Chiarelli¹, Nicole A. Grieshaber¹, Scott S. Grieshaber¹

¹Department of Biological Sciences, University of Idaho

This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.

Environment

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

Peter J. Wieber^1,2, Gabrielle Feber³, Patrick J. Hrdlicka¹, Zachary Kayler³

¹Department of Chemistry, University of Idaho, ²Department of Chemical and Biological Engineering, University of Idaho, ³Department of Soil and Water Systems, University of Idaho

A method for metabolomic analysis of barley is presented. The method entails fractionation and derivatization of metabolites and analysis thereof by gas chromatography/mass spectrometry (GC/MS). Metabolomic analysis can be used to determine the effect of intercropping and drought stress on the grain.