<ol>
<li>Mouse neural precursors (NPCs) were isolated from E12.5 embryo cortex. </li>
<li>Skin and mesenchymal layers were removed from dissected telencephalic vesicles. </li>
<li>Vesicles were incubated in 0.05% trypsin with 0.02% EDTA and 0.2% BSA in HBSS for 20 minutes at 37&deg;C.</li>
<li>Trypsinization was stopped by an equal volume of 1 mg/ml soybean trypsin inhibitor (Sigma #T6522) in HBSS. </li>
<li>Tissue digests were dissociated using several rounds of trituration with fire-polished Pasteur pipettes. </li>
<li>Cells were washed once ...

<ol>
	<li>Currle, D., Cheng, X., Hsu, C., Monuki, E., Flanagan, L., Rebaza, L., Derzic, S., Schwartz, P., Monuki, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+and+indirect+roles+of+CNS+dorsal+midline+cells+in+choroid+plexus+epithelial+formation.">Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelial formation.</a> <em style='font-style: italic'>Development</em>. <b>132</b> (15), 3549-3559 (2005).</li></ol>

<p class="jove_content">Great advances in our understanding of CNS development and stem cell biology have been made possible by our ability to harvest, isolate and culture embryonic neural stem cells.  This video demonstrates the dissection of  E12.5 mouse cerebral cortex and the subsequent disaggregation and culturing of embryonic neural stem cells.  Many other other similar methods have been successfully employed by other investigators.</p>

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culture of mouse neural stem cell precursors

Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase our understanding of the nervous system and may allow us to develop treatments for currently incurable brain diseases and injuries. In addition, stem cells should be used for stem cell research aimed at the detailed study of mechanisms of neural differentiation and transdifferentiation and the genetic and environmental signals that direct the specialization of the cells into particular cell types.  This video demonstrates a technique used to disaggregate cells from the embryonic day 12.5 mouse dorsal forebrain. The dissection procedure includes harvesting E12.5 mouse embryos from the uterus, removing the "skin" with fine dissecting forceps and finally isolating pieces of cerebral cortex. Following the dissection, the tissue is digested and mechanically dissociated. The resuspended dissociated cells are then cultured in "stem cell" media that favors growth of neural stem cells.

Watch this Scientific Journal Video about Culture of Mouse Neural Stem Cell Precursors at JoVE.com

Culture of Mouse Neural Stem Cell Precursors

<p class="jove_content">This video describes the method used for isolation of neuroprecursors from the developing cortex of embryonic mice.  The procedure for removing embryos from the uterus, dissecting the cortical tissue, and digesting the isolated cerebral cortex is shown.</p>

Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase ...

culture-of-mouse-neural-stem-cell-precursors

Research

JoVE Journal

Biology

19.8K 조회수.  University of California, Irvine (UCI). 이 동영상은 배아 생쥐의 개발​​ 피질에서 neuroprecursors의 절연에 사용되는 방법을 설명합니다. , 자궁에서 태아를 제거하는 대뇌 피질의 조직을 해부하고, 고립 대뇌 피질을 소화에 대한 절차는 표시됩니다.