Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

0:20

Isolating bone marrow cells

0:21

Dissecting mouse to isolate bones

1:03

Isolating tibia

1:40

Isolating femur

2:12

Isolating hip bone

2:50

Isolating spine

4:07

Cleaning bones

6:37

Crushing bones

9:33

Lineage depletion of bone marrow cells

14:06

Antibody staining for FACS

16:06

Transplantation into irradiated mouse

18:50

Discussion