Name: isolation and transplantation of hematopoietic stem cells (hscs)
Uploaded: 2007-02-25T00:00:00
Description: Watch this Scientific Journal Video about Isolation and Transplantation of Hematopoietic Stem Cells HSCs at JoVE.com

<p class="jove_title">Total bone marrow preparation</p>
<ol>
<li>Four&nbsp;B6 mice are sacrificed and dissected to obtain tibias, femurs, hip and spine.</li>
<li>During the dissections, the limbs and the bones are kept in PBS 2%FCS heat inactivated (optional 2mM EDA - dissection medium).</li>
<li>The clean bones are crushed with mortar and pestle in dissection medium. An efficient way to do it is to crush tibias, femurs and hips of each mouse, then 2 spines at a time. </li>
<li>The cell mixture obtained from each mouse is kept separate and filt...

isolation and transplantation of hematopoietic stem cells (hscs)

Watch this Scientific Journal Video about Isolation and Transplantation of Hematopoietic Stem Cells HSCs at JoVE.com

Isolation and Transplantation of Hematopoietic Stem Cells HSCs

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Research

JoVE Journal

Biology

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny.  Embryonic stem cells (ESC) are ...

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Hematopoietic stem cells (HSCs) have the ability to self-renew and generate all cell types of the blood lineages throughout the lifetime of an individual. ...

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

In the mouse embryo, early hematopoiesis occurs simultaneously in multiple organs, which includes the yolk sac and aorta-gonad-mesonephros region. These ...

Generation of Human CD40-activated B cells

CD40-activated B cells (CD40-B cells) have been identified as an alternative source of immuno-stimulatory antigen-presenting cells (APC) for cancer ...

Isolation and Derivation of Mouse Embryonic Germinal Cells

The ability of embryonic germinal cells (EG) to differentiate into primordial germinal cells (PGCs) and later into gametes during early developmental ...

Lentiviral-mediated Knockdown During <em>Ex Vivo</em> Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Erythropoiesis is a commonly used model system to study cell differentiation. During erythropoiesis, pluripotent adult human hematopoietic stem cells ...

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

The bone marrow is the principal site where HSCs and more mature blood cells lineage progenitors reside and differentiate in an adult organism. HSCs ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Muscle satellite cells are a stem cell population required for postnatal skeletal muscle development and regeneration, accounting for 2-5% of sublaminal ...

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

As cellular metabolism is a key regulator of hematopoietic stem cell (HSC) self-renewal, the various roles played by the mitochondria in hematopoietic ...