분할 Ubiquitin 기반 막 효모 두 하이브리드 (신화) 시스템 : 단백질 단백질 상호 작용을 확인을위한 강력한 도구

1:22

Introduction

1:41

Selection of Bait and Appropriate MYTH System

3:07

a. Vector Preparation and Amplification of Gene of Interest

5:30

b. Yeast Transformation and Recovery of Bait Plasmid DNA

8:10

MYTH Screening

7:03

Bait Validation

3:04

Generation of tMYTH Baits by Gap Repair

8:12

a. Large Scale Yeast Transformation

11:31

b. Bait Dependency Test

12:54

Representative Results/ Outcome

13:12

Conclusion