September 2nd, 2012
•Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography.
Tags
Related Videos
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
Crystallization of Membrane Proteins in Lipidic Mesophases
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
Copyright © 2024 MyJoVE Corporation. 판권 소유