The authors declare that they have no competing financial interests.

동물은 장벽 무균 시설에서 보관되어 있습니다. 모든 동물은 국가에 의해 의료 연구를위한 국가 사회 &quot;및&quot;국립 과학 아카데미에 의해 제조 실험 동물 &quot;의 관리 및 사용을위한 설명서에 의해 공식화 및 출판&quot;실험 동물 관리의 주체 &quot;을 준수하여 인간의 서비스를받을 수 보건원 (NIH 공개 NO. 86-23은 1985 년 개정). 모든 실험은 교육 과학 문화의 오스트리아 교육부에 의해 승인됩니다. 1받는 사람 준비 <ol><li> 자일 라진 (xylazine) (5 ㎎ / ㎏ 체중) 및 케타민 (100 ㎎ / ㎏ 체중)의 IM 주사받는 동물을 마취. </li><li> 동물의 측면 자궁 지역의 모든 머리를 제거하고 수술 필드에게 chlorhexdine를 사용하여 세 번 닦습니다. </li><li> 수술 필드에서 누운 자세에서 마우스를 놓습니다. </li><li> 다음 경정맥 포함에서 피부 절개를오른쪽 아랫 부리에 ision. </li><li> 그 후, 퉁명스럽게 오른쪽 외부 경정맥 (EJV)을 동원 합자 사이의 가지를 나눕니다. </li><li> 그런 다음 커프 몸 근위 그루터기 에버트하기에 충분한 길이를 떠날 확인하는 합자 사이의 EJV를 나눕니다. </li><li> 다음 악하선의 오른쪽 엽을 제거합니다. </li><li> 정맥 문합를 들어 폴리에틸렌 커프를 통해 EJV의 기단부를 통과하고 마이크로 클램프 커프의 핸들에서 문제를 해결할. </li><li> , 용기의 끝 부분에있는 합자를 제거 커프를 통해 루멘 에버트와 8-0 실크 루프 (그림 1a)로 고정한다. </li><li> 그 후 경동맥에 액세스 할 양극성 소작과 오른쪽 흉쇄 근육을 가로로 쪼개다. </li><li> 다음 경동맥을 동원하고 합자 사이의 혈관을 잘라. </li><li> 다음 경동맥을 동원하고 합자 사이의 혈관을 잘라. </li><li> DIS 합격탈의 팔목을 통해 용기의 끝은 동맥 클램프로 고정한다. </li><li> 용기의 단부에 봉합사를 제거하고, 혈관을 사용 dilatators 루멘 넓히다. 다음에, EJV 유사하게, 커프 위에 동맥 에버트 및 8-0 실크 루프로 고정한다. </li></ol> 2 심장 조달 <ol><li> 자일 라진 (xylazine) (5 ㎎ / ㎏ 체중) 및 케타민 (100 ㎎ / ㎏ 체중)의 IM 주사 공여 마우스를 마취. 그리고 동물의 복부의 모든 머리를 제거합니다. chlorhexdine를 사용하여 수술 필드에 세 시간을 스크럽. 수술 필드에서 앙와위에서 마우스 전술 된 바와 같이 다음 장소. </li><li> 중간 선 복부 절개에 따라, 하대 정맥 (IVC)를 노출하기 위해 왼쪽에 Q-팁을 내장을 철회. </li><li> 헤파린에 대한 IVC에 4 헤파린 나트륨 - 솔루션 : 1의 400 μl를 주입한다. </li><li> ACC을 얻을 수 cranially를 통해 전방 흉벽을 개흉술을 수행하고 배심장에 ESS. </li><li> 다음에, 흉선을 제거한 좌우 우수한 대정맥의 절개를 배출하게. </li><li> 그 후, 완두 분기의 수준에서 27 G 주사기와 대동맥 삽관에 의해 4 섭씨 HTK 용액과 역행 방식으로 마음을 관류. </li><li> 8-0 실크 열등한 우수한 대정 맥을 결찰과 합자로 원심을 나눕니다. </li><li> 다음 이전 삽관의 수준에서 대동맥 궁을 분할하고 폐동맥을 무료로 해부하고 가능한 한 멀리 원심을 나눕니다. </li><li> 그 후, 대량의 결찰 폐 혈관을 묶고 합자로 원심을 나눕니다. </li><li> 마지막으로, 공여 부위에서 심장을 제거하고 4 ° 섭씨 HTK 용액 (그림 1b)에 보관합니다. </li></ol> 3 주입 <ol><li> 거꾸로 위치에받는 사람의 목 지역에서 심장 이식을 배치 (그림 1C </strong&gt;). </li><li> 다음받는 사람의 정맥 커프 구조를 통해 심장의 폐동맥을 끌어와 실크 루프 (그림 1D)로 고정한다. </li><li> 이식편의 대동맥과 같은 방식 (그림 1E)에서받는 동물의 everted 동맥 사이의 문합을 수행합니다. </li><li> 이어서, 동맥 클램프이어서 제 정맥 클램프를 제거. 심장 재관류하고 1 ~ 2 분 이내에 치기 시작합니다. 재관류 동안 따뜻한 (35 ° 섭씨) 식염수로 마음을 적 십니다. </li><li> 마지막으로 6-0 연속 봉합 (그림 1E)와 수술 상처를 닫습니다. </li></ol> (4) 수술 후 관리, 엔드 포인트 <ol><li> 유체 교체 수술 후 0.3 ml의 생리 식염수의 IP를 부여합니다. </li><li> 마취에서 각성 할 때까지 가열 램프 아래에있는 동물을 놓습니다. </li><li> 깨어 나면, 그들은 물과식이를받을 주거 시설에 동물을 반환합니다. </li><li> MI하려면제 7 수술 후 일 동안 nimize 통증 carprofen (4 ㎎ / kg 매 12 시간의 피하 (SC)를 관리 할 수​​ 있습니다. 또한, 오일에 바로 수술 후) Buprenorphin마다 12 시간을 (0.1 ㎎ / ㎏을 제공합니다. </li><li> 적절한 영양 섭취에 대해 평가하기 위해 각받는 사람 동물 주간의 무게 (g)을 얻었다. 이상 ~ 15 %의 체중 감소의 증거가 수술 최신 무관심 마비 또는 매우 구부러진 뒤쪽에 비해 중량이있는 경우, 임상 적 종점에 도달하기 전에 단말기 흡입 이소 플루 란을 이용하여 동물을 희생. 심장이 거부 된 후에 또한 동물을 희생. </li></ol>

<ol>
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This study has been supported by grant #207 of the &#8220;Medizinischer Forschungsfond Tirol&#8221;, grant #2010022010 of the &#8220;MUI-START F&#246;rderungsprogramm&#8221; of the Innsbruck Medical University.

혈관 이식의 거부는 충분히 체외 모델에서 사용하여 평가 될 수있는 다른 단계의 과다를 수반한다. 받는 사람의 감작 항원 처리 이차 림프 기관에서뿐만 아니라 면역 능력 세포의 분화 및 증식 같은 현상은 생체 내에서 특성화 수 있습니다. 동물 모델함으로써 중개 연구 (16)을위한 이상적인 도구입니다. 마우스 모델은 여전히 형질 전환 유전자 녹아웃 마우스의 넓은 범위 때?...

심장 이식은 다른 말기 심장 질환으로 고생하고있는 환자들을위한 선택의 치료를 나타냅니다. 수술 기법, 감염의 더 효과적인 예방 및 새로운 면역 억제 요법의 발전은 장기 이식 6에서 현저하게 향상된 결과를 초래. 그러나, 장기 이식 생존율은 지난 7 년 동안 엄격하게 개선되지 않았습니다. 이식 동맥 경화에 의해 특징 만성 거부, 장기 이식 생존 8-11에 큰 장애물이되고 있습니다. 생쥐 이소성 심장 이식 모델은 급성 동안 면역 학적 메커니즘의 분석을위한 중요하고 유효한 도구뿐만 아니라 만성 거부 반응 12-15을 제공합니다. 현재까지 가장 흔한 이식 모델은 봉합 기술을 사용하여 복부 마우스 심장 이식은 여전히​​있다. 기증자 심장의 대동맥이에 문합된다bdominal 대동맥과 폐동맥받는 사람의 하대 정맥에 문합된다. 봉합 모델 미세 어려움의 대부분은 16, 17을 봉합 용기의 작은 크기에 기초한다. 봉합 모델 달리 심장 외부 경정맥은 폐동맥 및 경동맥 기증자의 대동맥에 문합되는 수신자의 목 영역에 배치된다. 커프 기술을 사용하여 자궁 심장 이식 모델의 개발의 이론적 근거는이 모델의 광범위한 응용을 허용 기본적인 미세 기술로 높은 성공률을 달성 할 수 있도록 동물 모델을 가지고 있었다. 이 방법의 주요 이점은 봉합 모델 (18)에 비해 출혈 및 혈전증과 같은 합병증을 관련 현저히 적게 발생 문합이다.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Yasargil Clip Mini Permanent 7mm</td>
			<td>Aesculap</td>
			<td>FE720K</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro vessel clip</td>
			<td>S &#38; T</td>
			<td>B1 00396 V</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscissor</td>
			<td>FST</td>
			<td>14075-11</td>
			<td></td>
		</tr>
		<tr>
			<td rowspan="2">Vesseldilatator</td>
			<td rowspan="2">S &#38; T</td>
			<td rowspan="2">D-5a.2 00125</td>
			<td></td>
		</tr>
		<tr>
			<td></td>
		</tr>
		<tr>
			<td>Microforceps</td>
			<td>FST</td>
			<td>Dumont 11271- 30</td>
			<td></td>
		</tr>
		<tr>
			<td>Clipapplicator</td>
			<td>S &#38; T</td>
			<td>CAF-4 00072</td>
			<td></td>
		</tr>
		<tr>
			<td>Microvessel clip</td>
			<td>S &#38; T</td>
			<td>B1 00396 V</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine cervical heart transplantation model using a modified cuff technique

<html> 마우스 모델은 단일 클론 항체의 다양한 이후 연구에 특별한 관심의 상업적 근친 정의 녹아웃 균주의 생체 내 (in vivo) 연구 기계적인 수행 할 수 있습니다. 봉합 기술을 사용하여 심장 이식 모델을 처음 성공적으로 쥐에서 개발 된 반면, 동일하게 널리 사용되는 쥐의 동등한로 번역이 미세 절차의 기술적 복잡성으로 인해 실현되지 않았습니다. 대조적으로, 또한, 쥐의 초기 개발 비 봉합 커프 기술들이 성공적으로 생쥐 1-3에서 사용하기에 적합 하였다. 혈관 재 형성을위한이 기술은 폴리에틸렌 커프 위에받는 용기 everting 개의 주요 단계 I)을 포함한다; II) 이전 everted받는 용기를 통해 기증자 용기를 당기고 원주 넥타이와 장소에 들고. 이것은 내피 층, 짧은 동작 시간 및 매우 높은 개존율 (4)의 연속성을 보장한다. t는 &quot;&gt; 혈관 문합을 위해이 기술을 사용하여 우리는 경동맥 유입 동맥를 들어. 95 %의 성공률로 1,000 명 이상의 자궁 심장 이식을 수행 근위 대동맥 궁은​​ 이식 된 심장의 역행 관류의 결과로 문합 하였다. 정맥 배수 이식의 폐동맥받는 사람 (5)의 외부 경정맥과 문합 하였다. 여기서, 우리는 비디오를 보충하기위한이 기술의 추가 정보를 제공합니다.

그림 2는 완전히 MHC 일치하지 않는 C57BL / 6받는 사람에 이식 후 BALB / C의 기증자로부터 심장 이식을 대표하는 생존 데이터를 보여줍니다. 마음은 이식 후 일 7 ± 1에 거부됩니다. 이식 된 동종의 손가락 촉진은 동종 이식 거부 반응의 시간 의존적 과정을 감지하는 중요한 방법이다. 우리의 시리즈에서 수행되는 모든 동계 심장 이식 무기한 생존 (&gt; 1백50일). <p class="jove_content" fo:keep-t...

Watch this Scientific Journal Video about Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique at JoVE.com

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

뮤린 자궁 심장 이식 모델은, 면역뿐만 아니라 허혈 재관류 손상 연구에 매우 적합하다. 우리는 비 봉합 커프 기술을 사용하여 절차를 수정이 방법으로 1,000 개 이상의 성공적인 이식을 수행 하였다. 여기서, 우리는 비디오를 보충하기위한이 기술의 추가 정보를 제공합니다.

수정 커프 기술을 사용하여 쥐의 자궁 심장 이식 모델

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

murine-cervical-heart-transplantation-model-using-modified-cuff

Research

JoVE Journal

Medicine

13.7K 조회수.  Innsbruck Medical University.  뮤린 자궁 심장 이식 모델은, 면역뿐만 아니라 허혈 재관류 손상 연구에 매우 적합하다. 우리는 비 봉합 커프 기술을 사용하여 절차를 수정이 방법으로 1,000 개 이상의 성공적인 이식을 수행 하였다. 여기서, 우리는 비디오를 보충하기위한이 기술의 추가 정보를 제공합니다. 

비디오: Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Murine Model of Wound Healing

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

상처 치유의 생쥐 모델

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

연구

의학

Murine Heterotopic Heart Transplant Technique

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique was developed and reported in 2001 by Niimi. Described here are the techniques that have evolved over more than a decade in the hands of three surgeons (Plenter, Grazia, Pietra) in our center. These techniques are now being passed on to a younger generation of surgeons and researchers.
Based largely on the Niimi experience, the procedures used have evolved in the fine details - details which we will endeavor to relate here in such a way that others may be able to use this very useful model. Like Niimi, we have found that a video aid to learning is a priceless resource for the beginner.

The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.

쥐 이소성 심장 이식 기술

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient&#8217;s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

이소성 심장 이식 마우스 모델을 사용하여 폐동맥 판막 이식

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

쥐의 각막 이식 : 고체 장기 이식의 가장 일반적인 형태를 연구하기위한 모델

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Heterotopic Cervical Heart Transplantation in Mice

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient&#8217;s common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.

The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

마우스의 이소성 자궁 심장 이식

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the aorta in mice using the sleeve method was short and easy averaging 20 min, permitting studies of iso/allo grafts. The following article describes the aortic transplantation procedure used in our laboratory. The mice were anesthetized with a mixture of 1.5% volume isoflurane and 100% oxygen through a face mask. At this point, the segment of the aorta between the renal arteries and its bifurcation was separated from the vena cava, freely prepared and clampedat the proximal and distal segments with a single silk suture. Prior to the removal of the aorta, a saline solution containing heparin was injected into the inferior vena cava. Then the aorta was cut between the clamps and a saline heparin solution was used to flush the lumen. The sleeve technique with monofilament sutures was used in order to transplant the abdominal aorta in the orthotopic position.

We present an orthotopic aortic transplantation model using the sleeve technique in mice. It is a very rapid anastomosis method, which can be employed in studies of vascular disease.

대동맥 이식-교육용 비디오의 마우스 모델에서 소매 기법을 사용 하 여

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the availability of the widest range of molecular probes and reagents to perform in vivo as well as in vitro studies. Based on our experience with various murine transplantation models, we developed a heterotopic pancreas transplantation model in mice with the intent to analyze mechanisms underlying severe ischemia reperfusion injury-associated early graft damage. In contrast to previously described techniques using suture techniques, herein we describe a new procedure using a non-suture cuff technique. 
In recent years, we have performed more than 300 pancreas transplantations in mice with an overall success rate of &gt;90%, a success rate never described before in mouse pancreas transplantation. The backbone of this non-suture cuff technique for graft revascularization consists of two major steps: (I) pulling the recipient vessel over a polyethylene/polyamide cuff and fixing it with a circumferential ligature, and (II) placing the donor vessel over the everted recipient vessel and fixing it with a second circumferential ligature. The resultant continuity of the endothelial layer results in less thrombogenic lesions with high patency rates and, finally, high success rates.
In this model, arterial anastomosis is achieved by pulling the abdominal aorta of the donor graft over the everted common carotid artery of the recipient animal. Venous drainage of the graft is achieved by pulling the portal vein of the graft over the everted external jugular vein of the recipient. This manuscript provides details and crucial steps of the organ recovery and organ implantation procedures, which will allow researchers with microsurgical skills to perform the transplantation successfully in their laboratories.

Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.

췌 장 이식 수정된 팔목 기술을 사용 하 여 마우스 모델

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

정형외 심장 이식의 전 임상 돼지 모델

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. However, only minor improvement in the long-term results of transplanted solid organs could be observed over the past decades. In this context, chronic allograft vasculopathy (CAV) still represents the leading cause of late organ failure in cardiac, renal and pulmonary transplantation.
Thus far, the underlying pathogenesis of CAV development remains unclear, explaining why effective treatment strategies are presently missing and emphasizing a need for relevant experimental models in order to study the underlying pathophysiology leading to CAV formation. The following protocol describes a murine heterotopic cervical aortic transplantation model using a modified non-suture cuff technique. In this technique, a segment of the thoracic aorta is interpositioned in the right common carotid artery. With the use of the non-suture cuff technique, an easy to learn and reproducible model can be established, minimizing the possible heterogeneity of sutured vascular micro anastomoses.

Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its formation.

수정된 비봉합사 커프 기술을 사용한 뮤린 자궁 경부 대동맥 이식 모델

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. ...

A Simplified Model for Heterotopic Heart Valve Transplantation in Rodents

There is an urgent clinical need for heart valve replacements that can grow in children. Heart valve transplantation is proposed as a new type of transplant with the potential to deliver durable heart valves capable of somatic growth with no requirement for anticoagulation. However, the immunobiology of heart valve transplants remains unexplored, highlighting the need for animal models to study this new type of transplant. Previous rat models for heterotopic aortic valve transplantation into the abdominal aorta have been described, though they are technically challenging and costly. For addressing this challenge, a renal subcapsular transplant model was developed in rodents as a practical and more straightforward method for studying heart valve transplant immunobiology. In this model, a single aortic valve leaflet is harvested and inserted into the renal subcapsular space. The kidney is easily accessible, and the transplanted tissue is securely contained in a subcapsular space that is well vascularized and can accommodate a variety of tissue sizes. Furthermore, because a single rat can provide three donor aortic leaflets and a single kidney can provide multiple sites for transplanted tissue, fewer rats are required for a given study. Here, the transplantation technique is described, providing a significant step forward in studying the transplant immunology of heart valve transplantation.

This protocol describes a simple and efficient method for the transplantation of aortic valve leaflets under the renal capsule to allow for the study of alloreactivity of heart valves.