November 12th, 2013
•Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
Related Videos
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
Isolation and Culture of Hippocampal Neurons from Prenatal Mice
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
Primary Culture of Mouse Dopaminergic Neurons
Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
Low-Density Primary Hippocampal Neuron Culture
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
Copyright © 2024 MyJoVE Corporation. 판권 소유