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Calcium Phosphate Transfection of Primary Hippocampal Neurons

DOI :

10.3791/50808-v

November 12th, 2013

November 12th, 2013

24,657 Views

1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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Calcium Phosphate Precipitation

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