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JoVE Journal

Bioengineering

This content is Open Access.

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

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12:01 min

October 19th, 2014

October 19th, 2014

18,044 Views

0:05

Title

1:48

Preparations

3:54

Calibrating the Settings

5:20

Measuring the Calibration and Sample Particles

7:32

Data Analysis

8:15

Alternative Approach to Measuring Non-purified Extracellular Vesicles

9:58

Results: U87-MG/EGERvIII Extracellular Vesicle Characterization

11:03

Conclusion

Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.

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