February 6th, 2015
•The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.
Related Videos
Laser Capture Microdissection of Drosophila Peripheral Neurons
Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
Copyright © 2024 MyJoVE Corporation. 판권 소유