January 31st, 2015
•We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
Tags
Related Videos
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
Copyright © 2024 MyJoVE Corporation. 판권 소유