Liberate Cells from Alginate and Actin Cytoskeleton Arrangement
5:54
Results: Human Pituitary Adenoma Cells in Three-dimensional Aliginate Bead Culture
7:09
Conclusion
필기록
The overall goal of this method, is to describe a three-dimensional alginate-bead culture for maintaining human pituitary adenoma cells. This method can help answer key questions in the tumor cell biology field, such as the expression of different
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Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.