Ligation of Linker Oligonucleotide to Fragmented Genomic DNA
6:58
Amplification of Viral LTR-host Genomic DNA Junctions by Semi-nested PCR
8:02
Results: Sequence Logos Depicting HIV-1 Base Preferences from Representative Experiment Libraries
8:52
Conclusion
필기록
The overall goal of this procedure is to PCR amplify retroviral integration sites from bulk genomic DNA, and sequence the resulting DNA library, such that precise integration locations can be mapped to a reference genome. This method can help answ
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We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using BEDTools.