DNA Elution, Cross-link Reversal and Proteinase K Digestion
7:33
DNA Isolation Using DNA-binding Beads
10:05
Results: ChIP Protocols Investigate the Epigenetic Regulation of Cardiac Gene Transcription
11:47
Conclusion
필기록
The overall goal of applying these chromatin immunoprecipitation approaches to differentiating embryonic stem cells or cardiac embryonic tissues, is to uncover the epigenetic regulation of cardiac development. Since fine tuning of gene transcripti
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A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.