Set up of Automated Protocols for Protoplast Isolation and Transformation
5:16
Automated Protocols
6:37
Microscopic Analysis of Transformation
7:42
Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts
9:40
Conclusion
필기록
The overall goal of this protocol is to develop a method for rapid, automated high-throughput screening of plant protoplasts, specifically to address the current bottleneck in early screening of large numbers of genome editing and gene silencing t
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A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.