Bacterial Artificial Chromosome (BAC) Recombineering of IL-22-tdTomato Vector Generation
3:22
Spleen, Thymus, Lymph Node, and Peyer's Patch Single Cell Suspension Generation
4:48
Intestinal Intraepithelial Lymphocyte and Lamina Propria Cell Isolation
6:40
IL-22-tdTomato Colitis Expression
7:57
Results: Representative Visualization and Localization of IL-22 Receptors
9:31
Conclusion
필기록
The overall goal of this mouse genetic modification procedure is to identify, purify, and characterize IL-22-producing immune cells. Cells of the immune system release mediators called cytokines which are responsible for defending us against forei
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We describe here a transgenic reporter mouse model to visualize the IL-22-producing cells inside different mouse tissues. This method can be used to track the location of other cytokines or secretary proteins in the mouse.