Real Time Intracellular Growth and Eukaryotic Cell Cytotoxicity Assay
3:14
Preparing Luminescent or Fluorescent Legionella pneumophila and Macrophage Infection
4:49
Assay Readout, and Single and Multi-dimensional Dose-response Testing and Data Interpretation
6:09
Results: Simultaneous Real-time Assay Results for Intracellular Bacterial Growth and Eukaryotic Cell Cytotoxicity
7:41
Conclusion
필기록
The overall goal of this protocol is to use a single combination real-time assay to identify specific small molecule inhibitors of intracellular bacterial growth that are non-toxic to host cells. This method can help answer key questions in the an
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A high throughput, real-time assay was developed to simultaneously identify (1) eukaryotic cell-penetrant antimicrobials targeting an intracellular bacterial pathogen, and (2) assess eukaryotic cell cytotoxicity. A variation on the same technology was thereafter combined with digital dispensing technology to enable facile, high-resolution, dose-response, and two- and three-dimensional synergy studies.