Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function
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Conclusion
필기록
The overall goals of this protocol are to collect conditioned medium from mouse embryonic stem cells under serum and feeder free culture conditions and to characterize the biological functions of this medium. This procedure provides insights into
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This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.