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JoVE Journal

Developmental Biology

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors

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08:01 min

February 28th, 2017

February 28th, 2017

7,730 Views

0:05

Title

0:51

Bone Marrow Cell Isolation

2:45

Osteoclast Differentiation

3:55

Jagged1-coated Surface Continuous and Temperary Notch Signaling Stimulation

6:08

Results: Representative Effects of Jagged1-Fc Signaling on Osteoclasts Precursor Gene Expression

7:24

Conclusion

필기록

The overall goal of this procedure is to observe the affects of notch signaling stimulation at varying points during the osteoclast differentiation process. This method can help answer key questions in the field of cell biology, such as:How does t

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Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.

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