Preparation of the Latex Cap and Intraventricular Balloon
3:06
Isolation and Cannulation of the Heart
4:34
Reversed-heart Model
6:24
Results: Protein and Purine Analysis of Cardiac Transudate
7:33
Conclusion
필기록
The overall goal of this inverted heart model is to collect cardiac interstitial transudate from an isolate rat heart. Within the beating heart, the individual sense of the heart release paracrine factors and they release those into the surroundin
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This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.