The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

1:24

Introduction to the Accompanying Publication

2:47

Nematode Fixation

4:19

Nematode Antibody Staining

6:33

Applying RNAi to Nematodes Using a Feeding Protocol

7:57

Analyzing Phenotypes Using Fluorescence Dissecting Microscopy

8:41

Analyzing Phenotypes Using Confocal Microscopy

10:14

Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis

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Conclusion