Results: Nitrosamide Standard Preparation and In Vitro Incubation
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Conclusion
필기록
The overall goal of this methodology is to determine if Cytochrome P450s can processively oxidize n nitrosamines to n nitosamides in vitro. This method benefits the toxicology field by evaluating if nitosamines are directly metabolized to nitrosam
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α-hydroxylation of carcinogenic nitrosamines by cytochrome P450s is the accepted metabolic pathway that produces DNA-damaging intermediates, which cause mutations. However, new data indicates further oxidation to nitrosamides can occur. We describe a general method for detecting nitrosamides produced from in vitro cytochrome P450-catalyzed metabolism of nitrosamines.