Name: re-arterialized rat partial liver transplantation with an in vivo vessel-oriented 70% hepatectomy
Uploaded: 2018-04-08T13:00:00.000+00:00
Duration: 14 min 52 s
Description: Watch this Scientific Journal Video about Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy at JoVE.com

이 연구는 국립 자연 과학 재단의 중국 (보조금 번호 81501382)와 절 강 지방 자연 과학 재단의 중국 (부여 번호에 의해 지원 되었다 LQ13H100003 그리고 LQ16H100002)입니다.

<ol>
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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Ophthalmic Microsurgical Suturing Techniques. , 21-28 (2007).</li><li>Delriviere, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detailed+modified+technique+for+safer+harvesting+and+preparation+of+liver+graft+in+the+rat.">Detailed modified technique for safer harvesting and preparation of liver graft in the rat.</a> Microsurgery. 17, 690-696 (1996).</li><li>Oldani, G., Lacotte, S., Morel, P., Mentha, G., Toso, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+rats.">Orthotopic liver transplantation in rats.</a> J Vis Exp. , (2012).</li><li>Delriviere, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Technical+details+for+safer+venous+and+biliary+anastomoses+for+liver+transplantation+in+the+rat.">Technical details for safer venous and biliary anastomoses for liver transplantation in the rat.</a> Microsurgery. 18, 12-18 (1998).</li><li>Jin, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preliminary+experience+of+a+PDCA-cycle+and+quality+management+based+training+curriculum+for+rat+liver+transplantation.">Preliminary experience of a PDCA-cycle and quality management based training curriculum for rat liver transplantation.</a> J Surg Res. 176, 409-422 (2012).</li><li>Selzner, N., Selzner, M., Tian, Y., Kadry, Z., Clavien, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cold+ischemia+decreases+liver+regeneration+after+partial+liver+transplantation+in+the+rat:+A+TNF-alpha/IL-6-dependent+mechanism.">Cold ischemia decreases liver regeneration after partial liver transplantation in the rat: A TNF-alpha/IL-6-dependent mechanism.</a> Hepatology. 36, 812-818 (2002).</li><li>Tanaka, H., Hashizume, K., Enosawa, S., Suzuki, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successful+transplantation+of+a+20%+partial+liver+graft+in+rats:+a+technical+innovation.">Successful transplantation of a 20% partial liver graft in rats: a technical innovation.</a> J Surg Res. 110, 409-412 (2003).</li><li>Bockhorn, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Erythropoietin+treatment+improves+liver+regeneration+and+survival+in+rat+models+of+extended+liver+resection+and+living+donor+liver+transplantation.">Erythropoietin treatment improves liver regeneration and survival in rat models of extended liver resection and living donor liver transplantation.</a> Transplantation. 86, 1578-1585 (2008).</li><li>Martins, P. N., Neuhaus, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+anatomy+of+the+liver,+hepatic+vasculature+and+bile+ducts+in+the+rat.">Surgical anatomy of the liver, hepatic vasculature and bile ducts in the rat.</a> Liver Int. 27, 384-392 (2007).</li><li>Kamada, N., Calne, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+the+rat.+Technique+using+cuff+for+portal+vein+anastomosis+and+biliary+drainage.">Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage.</a> Transplantation. 28, 47-50 (1979).</li><li>Kashfi, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+various+techniques+of+orthotopic+liver+transplantation+in+the+rat.">A review of various techniques of orthotopic liver transplantation in the rat.</a> Transplant.Proc. 37, 185-188 (2005).</li><li>Wain, R. A. J., Hammond, D., McPhillips, M., Whitty, J. P. M., Ahmed, W., Ahmed, W., Jackson, M. 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모든 저자는 공개를 전혀 상반 되는 관심사를 있다.

쥐 PLTx 훈련 프로그램을 비용에서 높고 긴 기간20에서 정교한 microsurgical 절차 이다. 쥐 PLTx 프로토콜의 복잡성 연구를이 동물 모델을 사용 하지 못했습니다. 전체 크기 쥐 LTx와 비교 하면, 이식 절차의 도전 뿐만 아니라 작은 동물에서 간 절제술의 도전으로 쥐 PLTx는 microsurgeon를 제공 합니다. 따라서, 세부 사항에 전체 절차를 설명 하는 시각된 microsurgical 프로토콜이 복잡 한 모델을...

현재, 기증된 간 기다리는 환자의 수와 간 기증된 장기의 수 사이 큰 차이가 있다. 간 장기의 부족은 세계적인 문제 이다. 기증자의 수영장을 확장, 분할 간 이식 (LTx), 살아있는 기증자 LTx 부분 간 이식1로 사용 하 여 개발 되었다.
추가 부분 간 이식 (PLTx) 뒤에 메커니즘을 조사, 관련 동물 모델 설립된2,3,,45되었습니다. 쥐 PLTx에서 간 엽 있으며 절제 vivo에서 ex vivo LTx 살아있는 기증자의 상태를 모방 하 여 인간의 LTx, 각각, 분할. 시각 실험의 저널에 에서 발표 된 논문 50% 쥐 PLTx는 ex vivo hepatectomy5를 사용 하 여 관련 된 상세한 프로토콜을 제시. 그러나, vivo에서 hepatectomy와 쥐 PLTx 하지 아직 알려졌다 시각화 된 문학에서.
Vivo에서 및 비보 전 hepatectomies 차이 자체 hepatectomy 수행의 기술 또한 많은 외과 연구 쥐 PTLx를 사용 하 여 현재, PLTx. 결과 결정에 중요 한 역할 담당 모델, 간 엽 간 엽2,3,6,7,,89의 작은 꽃 자루에는 간단한 결 찰 후 절제 했다. 그러나, 다른 모양 및 크기는 간단한 결 찰 절제술 다른 간 엽으로 모든 간 엽에 적합 하지 않습니다 하기 전에 배치. 중간 엽의 기지에서 간단한 결 찰 결국 부분 간 이식10,11의 유출에 영향을 미칠 수 있습니다 베 나 정 맥의 수축이 발생의 높은 위험을 운반 합니다. 따라서, 쥐 간 해부학의 지식을 바탕으로 하는 업데이트 hepatectomy 기법 쥐 PTLx의 분야에서 필요 합니다.
이 연구에서 설명 하는 프로토콜에 업데이트 선박 지향 70 %hepatectomy 쥐 PLTx의 절차에 통합 되었다. 포털 정 맥과 왼쪽된 측면 엽 (LLL) 및 중간 엽 (ML)의 간 동맥 해 부 되었고 간 실질의 제거 하기 전에 개별적으로 분할. 그런 다음, LLL와 ML의 간장 혈관 봉합을 피어 싱으로 출혈 했다. 개별 ligations 여러 날카로운 봉합을 사용 하 여 보다 간단한 결 찰을 배치, 여는 ML의 나머지 그 루터 기 베 나 정 맥에 걸쳐 수 있었습니다. 따라서, infrahepatic 베 나 카바 수술 결 찰으로 인 한 수축 피할 수 있었다. 또한, 개별 ligations 간 실질의 감소는 간 남은 그 루터 기 실험11혈액 손실의 영향을 최소화에 출혈의 속도 전에 의해 LLL와 ML의 혈액 공급의 폐색.
Microsurgeons에 대 한이의 매우 작은 직경 때문에 간 이식의 적절 한 간 동맥 (PHA)를 재구성 하 중요 한 도전 이다. 간 동맥을 재구성을 위해 수많은 microsurgical 기술 제안된14 되었습니다 비록 LTx 재 arterialization 인지 진정으로 필요한의 질문은 아직 논쟁12,,1314, . 여기, 우리는 엔드-투-사이드 방식 확대 PHA를 anastomoses 일반적인 간 동맥 (차) 간 이식의 다시 arterialization에 대 한 새로운 기술을 소개 합니다. 이 엔드-투-사이드 기법을 사용 하 여 두 간 동맥 더 큰 직경의 문 합으로 연결 됩니다. 큰는 문 합의 직경은, 쉽게 손을 봉합 수행 이며 큰는 문 합의는 patency에 개선 된다.

<table><tbody><tr><td>Surgical microscope</td><td>M&amp;ouml;ller-Wedel, Germany</td><td>654359</td><td></td></tr><tr><td>Light source</td><td>OSRAM (China) Lighting Co., Ltd</td><td>64627</td><td></td></tr><tr><td>Isoflurane Vaporizer</td><td>MIDMARK Corporation</td><td>VIP3000</td><td></td></tr><tr><td>Isoflurane</td><td>Baxter Healthcare Corporation</td><td>40032609</td><td>For Inhalation anesthesia</td></tr><tr><td>Normal Saline</td><td>Zhejiang Tianrui Pharmaceutical Co., Ltd.</td><td>716092103</td><td></td></tr><tr><td>Povidone Iodine Solution</td><td>HANGZHOU MINSHENG PHARMACEUTICAL</td><td>H33021567</td><td>For disinfection</td></tr><tr><td>Hemostatic forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>J31020</td><td></td></tr><tr><td>Surgical scissors</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>JC2116</td><td></td></tr><tr><td>Needle-holder</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>J32030</td><td></td></tr><tr><td>Dressing Forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>J42020</td><td></td></tr><tr><td>Mosquito hemostatic forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>W40340</td><td>For liver resection; For retracting the stay sutures.&amp;nbsp;</td></tr><tr><td>Micro-scissors</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>WA1040</td><td></td></tr><tr><td>Micro needle-holder</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>WA2060</td><td></td></tr><tr><td>Curved micro typing forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>WA3061</td><td></td></tr><tr><td>straight micro typing forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>WA3060</td><td></td></tr><tr><td>Micro hemostatic forceps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>WA3020</td><td></td></tr><tr><td>Bulldog clamp</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>XEC350</td><td></td></tr><tr><td>Vessel clamps</td><td>Shanghai Medical Instruments(Group) Ltd</td><td>W40160</td><td></td></tr><tr><td>Micro Serrefine-curved</td><td>&amp;nbsp;Fine Science Tools, Inc</td><td>18055-05</td><td></td></tr><tr><td>Dacryosyrinx</td><td>Shi Zhuo medical instruments Co., Ltd.</td><td>5# curve</td><td>Use as the curved needle for forcing out the air bubbles in the vascular lumen</td></tr><tr><td>Sterilized gauze swabs</td><td>Fuqing Health &amp;amp; Integral Medical</td><td>20230R</td><td></td></tr><tr><td>5mL syringe</td><td>Zhejiang Yusheng Medical Instrument Co.,Ltd</td><td>811932416</td><td></td></tr><tr><td>18G needles</td><td>Shanghai Kindly Enterprise Development Group Co., Ltd</td><td>01-014</td><td>For fixing the upper abdomen wall</td></tr><tr><td>Intima II 22G intraveous catheter</td><td>Becton Dickinson Medical Devices (Shanghai) Co Ltd.</td><td>383207y</td><td>For making biliary stent; For infusing perfusate</td></tr><tr><td>7-0 polypropylene sutures</td><td>Ningbo Medical Needle Co., Ltd</td><td>151026</td><td>For SHVC anastomsis</td></tr><tr><td>6-0 silk suture</td><td>Shanghai Pudong Jinhuan Medical Products Co., Ltd</td><td>2650182</td><td>For ligation</td></tr><tr><td>Culture dish (100mm)</td><td>Corning Incorporated</td><td>430165</td><td>used in back table for storage ice. The back table dish was placed on it.&amp;nbsp;</td></tr><tr><td>Culture dish (30 mm)</td><td>Corning Incorporated</td><td>3296</td><td>As a vessel for organ preservation</td></tr><tr><td>Angiocath 12G intravenous catheter</td><td>Becton Dickinson Medical Devices (Shanghai) Co Ltd.</td><td>382277</td><td>For making a IHVC cuff</td></tr><tr><td>Angiocath 14G intravenous catheter</td><td>Becton Dickinson Medical Devices (Shanghai) Co Ltd.</td><td>382269</td><td>For making a PV cuff</td></tr><tr><td>Buprenorphine (hydrochloride)</td><td>Tianjin Institute of Pharmaceutical Research Pharmaceutical Co., Ltd</td><td>H12020275</td><td>For analgesic</td></tr><tr><td>Cefuroxime sodium for injection</td><td>Esseti FarmaceuticiS.r.l</td><td>H20160013</td><td>As an antibiotics</td></tr></tbody></table>

re-arterialized rat partial liver transplantation with an in vivo vessel-oriented 70% hepatectomy

분할된 간 이식 및 살아있는 간 기증자 간 이식 간 장기에서 더 효율적인 방법으로 활용 하는 병원에서 개발 되었다. 더 잘 이해 하려면 이러한 수술 절차 뒤에 메커니즘, 쥐 부분 간 이식 (PLTx) 모델 관련 수술 연구를 위해 설립 되었다. 쥐 PLTx 모델의 복잡성 때문에 자세한 설명과 함께 프로토콜이 필요 합니다. 게시 된 문서 이전 프로토콜 hepatectomy 비보 전 50% 쥐 PLTx를 달성 하기 위해 사용 되었다 보고. 이 프로토콜을 달리 vivo에서 70 %hepatectomy 다시 arterialized PLTx를 도입 했습니다. 업데이트 된 선박 지향 hepatectomy 쥐 microsurgical 절차를 수정 하기 위해 PLTx에 통합 되었다. 포털 정 맥과 간 동맥 왼쪽된 측면 엽과 중간 엽의 개별적으로 해 부 되었고 나머지 간 그 루터 기에서 출혈의 확률 감소는 간 실질의 제거 하기 전에 출혈. 또한, 일반적인 간 동맥 확대 적절 한 간 동맥 사이 엔드-투-사이드 혈관 문 합 간 동맥을 다시 arterialize을 소개 했다. 이 엔드-투-사이드 혈관 문 합 기법을 사용 하 여 그로 인하여 손 봉합의 어려움을 감소 하 고 anastomotic patency의 높은 속도 유지 하는 문 합의 직경 확대 했다. 또한, infrahepatic 베 나 정 맥의 갑 문 합을 약간 수정 했습니다. 받는 사람의 베 나 정 맥 주위 원주 간 실질의 섹션 갑 문 합 혈관 루멘의 3 차원 모양을 유지 하는 동안 보존 되었다. 간 실질의이 섹션은 문 합을 완료 한 후 제거 되었습니다. 이 수정, 숙박 봉합의 배치와 관련 된 단계 갑 문 합 시간을 단축 함으로써 추가 생략 되었다. 쥐 PLTx의이 프로토콜을 사용 하 여 낮은 간 효소 수준, 그대로 간 lobular 건축과 높은 생존 율은 미세 후 달성 했다.

총, syngeneic arterialized 쥐 PLTx의 31 경우가이 프로토콜을 사용 하 여 완료 되었습니다. 모든 받는 사람에 게 관찰 시간의 끝까지 살아남았다. 받는 사람의 몸 무게는 수술 후 다음날 (포드) 4 복구 하기 시작 했다. 몸 무게의 기울기 포드 6 (그림 8), 받는 사람의 복구를 직접 암시 후 일반 루이스 쥐의 가까이 이었다. 조직학, 담 즙 덕트의 약간의 확산은 받?...

Watch this Scientific Journal Video about Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy at JoVE.com

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

여기, 다시 arterialized 쥐 부분 간 이식 관련 프로토콜 제공 됩니다. 특히, 70% 간 선박 지향 hepatectomy의 업데이트 기술을 사용 하 여 절제 vivo에서 했다. 간 동맥은 엔드-투-사이드 방식으로 개축 되었다. 팔목 기술 infrahepatic 베 나 정 맥 문 합 시간을 수정 했습니다.

다시는 vivo에서 선박 지향 70% 쥐 부분 간 이식 Arterialized Hepatectomy

Split liver transplantation and living liver donor liver transplantation were developed in the clinic to utilize liver organs in a more efficient manner. ...

re-arterialized-rat-partial-liver-transplantation-with-an-vivo-vessel

Research

JoVE Journal

Medicine

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

11.5K 조회수.  The First Affiliated Hospital of Wenzhou Medical University. 여기, 다시 arterialized 쥐 부분 간 이식 관련 프로토콜 제공 됩니다. 특히, 70% 간 선박 지향 hepatectomy의 업데이트 기술을 사용 하 여 절제 vivo에서 했다. 간 동맥은 엔드-투-사이드 방식으로 개축 되었다. 팔목 기술 infrahepatic 베 나 정 맥 문 합 시간을 수정 했습니다.

비디오: Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Orthotopic Liver Transplantation in Rats

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat liver transplantation in 1979 by Kamada et al.3, this model has remained the gold standard despite various proposed alternative techniques4. Nevertheless, its broader use is limited by its steep learning curve5.
In this video paper, we show a simple and easy-to-establish revision of Kamada's two-cuff technique. The suprahepatic vena cava anastomosis is performed manually with a running suture, and the vena porta and infrahepatic vena cava anastomoses are performed utilizing a quick-linker cuff system6. Manufacturing the quick-linker kit is shown in a separate video paper.

We present an easy-to-establish revision of the classical two-cuff technique for orthotopic liver transplantation in rat.

쥐에게 Orthotopic 간 이식

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

연구

의학

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2, immunological responses 3,4, hemodynamics 5,6, and small-for-size syndrome 7. The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn. Consequently, the use of this model has been limited. Since the reliability and reproducibility of results are key components of the experiments in which such complex animal models are used, it is essential for surgeons who are involved in rat OLT to be trained in well-standardized and sophisticated procedures for this model.
While various techniques and modifications of OLT in rats have been reported 8 since the first model was described by Lee et al. 9 in 1973, the elimination of the hepatic arterial reconstruction 10 and the introduction of the cuff anastomosis technique by Kamada et al. 11 were a major advancement in this model, because they simplified the reconstruction procedures to a great degree. In the model by Kamada et al., the hepatic rearterialization was also eliminated. Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14.
In this article, we present detailed surgical procedures for a rat model of OLT with hepatic arterial reconstruction using a 50% partial graft after ex vivo liver resection. The reconstruction procedures for each vessel and the bile duct are performed by the following methods: a 7-0 polypropylene continuous suture for the supra- and infrahepatic vena cava; a cuff technique for the portal vein; and a stent technique for the hepatic artery and the bile duct.

Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

간 간선 재건과 부분 Orthotopic 간 이식의 쥐 모델에 대한 외과 절차

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure

In acute hepatic failure auxiliary liver transplantation is an interesting alternative approach. The aim is to provide a temporary support until the failing native liver has regenerated.1-3 The APOLT-method, the orthotopic implantation of auxiliary segments- averts most of the technical problems. However this method necessitates extensive resections of both the native liver and the graft.4 In 1998, Erhard developed the heterotopic auxiliary liver transplantation (HALT) utilizing portal vein arterialization (PVA) (Figure 1). This technique showed promising initial clinical results.5-6 We developed a HALT-technique with flow-regulated PVA in the rat to examine the influence of flow-regulated PVA on graft morphology and function (Figure 2).
A liver graft reduced to 30 % of its original size, was heterotopically implanted in the right renal region of the recipient after explantation of the right kidney.&#160; The infra-hepatic caval vein of the graft was anastomosed with the infrahepatic caval vein of the recipient. The arterialization of the donor&#8217;s portal vein was carried out via the recipient&#8217;s right renal artery with the stent technique. The blood-flow regulation of the arterialized portal vein was achieved with the use of a stent with an internal diameter of 0.3 mm. The celiac trunk of the graft was end-to-side anastomosed with the recipient&#8217;s aorta and the bile duct was implanted into the duodenum. A subtotal resection of the native liver was performed to induce acute hepatic failure. 7
In this manner 112 transplantations were performed. The perioperative survival rate was 90% and the 6-week survival rate was 80%. Six weeks after operation, the native liver regenerated, showing an increase in weight from 2.3&#177;0.8 g to 9.8&#177;1 g. At this time, the graft&#8217;s weight decreased from 3.3&#177;0.8 g to 2.3&#177;0.8 g.
We were able to obtain promising long-term results in terms of graft morphology and function. HALT with flow-regulated PVA reliably bridges acute hepatic failure until the native liver regenerates.

Auxiliary liver transplantation provides a temporary support in acute hepatic failure, until regeneration of the failing liver. The heterotopic auxiliary liver transplantation (HALT) with portal vein arterialization (PVA) renders sufficient liver function. We developed an analogous technique in the rat, to examine the influence of the portal vein arterialization on the morphology and function of the graft.

이소성 보조 쥐 간 이식 급성 간 장애에 포털 정맥 Arterialization를 유량 조절

In acute hepatic failure auxiliary liver transplantation is an interesting alternative approach. The aim is to provide a temporary support until the ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver engineering, including in vivo organ decellularization followed by repopulation, has emerged as a promising approach over ex vivo liver engineering. However, postoperative survival was not achieved. The aim of this study is to develop a novel surgical technique of in vivo selective liver lobe perfusion in rats as a prerequisite for in vivo liver engineering. We generate a circuit bypass only through the left lateral lobe. Then, the left lateral lobe is perfused with heparinized saline. The experiment is performed with 4 groups (n = 3 rats per group) based on different perfusion times of 20 min, 2 h, 3 h, and 4 h. Survival, as well as the macroscopically visible change of color and the histologically determined absence of blood cells in the portal triad and the sinusoids, is taken as an indicator for a successful model establishment. After selective perfusion of the left lateral lobe, we observe that the left lateral lobe, indeed, turned from red to faint yellow. In a histological assessment, no blood cells are visible in the branch of the portal vein, the central vein, and the sinusoids. The left lateral lobe turns red after reopening the blocked vessels. 12/12 rats survived the procedure for more than one week. We are the first to report a surgical model for in vivo single liver lobe perfusion with a long survival period of more than one week. In contrast to the previously published report, the most important advantage of the technique presented here is that perfusion of 70% of the liver is maintained throughout the whole procedure. The establishment of this technique provides a foundation for in vivo partial liver engineering in rats, including decellularization and recellularization.

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.

Vivo에서 부분 간 엔지니어링을에서 위한 쥐를 기초로 새로운 수술 기법

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

생체공학

Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice

A modified silicone injection procedure was used for visualization of the hepatic vascular tree. This procedure consisted of in-vivo injection of the silicone compound, via a 26 G catheter, into the portal or hepatic vein. After silicone injection, organs were explanted and prepared for ex-vivo micro-CT (&#181;CT) scanning. The silicone injection procedure is technically challenging. Achieving a successful outcome requires extensive microsurgical experience from the surgeon. One of the challenges of this procedure involves determining the adequate perfusion rate for the silicone compound. The perfusion rate for the silicone compound needs to be defined based on the hemodynamic of the vascular system of interest. Inappropriate perfusion rate can lead to an incomplete perfusion, artificial dilation and rupturing of vascular trees.
The 3D reconstruction of the vascular system was based on CT scans and was achieved using preclinical software such as HepaVision. The quality of the reconstructed vascular tree was directly related to the quality of silicone perfusion. Subsequently computed vascular parameters indicative of vascular growth, such as total vascular volume, were calculated based on the vascular reconstructions. Contrasting the vascular tree with silicone allowed for subsequent histological work-up of the specimen after &#181;CT scanning. The specimen can be subjected to serial sectioning, histological analysis and whole slide scanning, and thereafter to 3D reconstruction of the vascular trees based on histological images. This is the prerequisite for the detection of molecular events and their distribution with respect to the vascular tree. This modified silicone injection procedure can also be used to visualize and reconstruct the vascular systems of other organs. This technique has the potential to be extensively applied to studies concerning vascular anatomy and growth in various animal and disease models.

Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using &#181;CT and histological serial sections.

일반 마우스의 70 % 부분 간 절제술 후 혈관 및 실질 재생의 시각화

A modified silicone injection procedure was used for visualization of the hepatic vascular tree. This procedure consisted of in-vivo injection of the ...

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep learning curve. The introduction of the cuff technique for anastomosis of the portal vein (PV) and infrahepatic vena cava (IHVC) has significantly simplified the transplant procedure in rats. However, due to the short anterior wall of the recipients' suprahepatic vena cava (SHVC), the cuff technique is very difficult to use for the reconstruction of the SHVC. Most researchers in this field still use the hand-suture technique for SHVC reconstruction, which makes it the bottleneck step in rat orthotopic liver transplantation. The magnetic anastomosis technique (i.e., magnamosis) is a method of connecting two vessels using the attractive force between two magnets. Our recent study has shown that the magnetic anastomosis technique is superior to the hand-suture technique for SHVC reconstruction in rats. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using the novel magnetic anastomosis technique. In this model, the reconstruction of the PV and IHVC was performed by the standard cuff technique, while the reconstruction of the bile duct (BD) was performed by a stent technique. The hepatic re-arterialization was not performed. The magnetic anastomosis technique made SHVC reconstruction much easier and significantly shortened the anphepatic phase. After a reasonable learning curve, even researchers without advanced microsurgical skills can produce reliable and reproducible results using this rat model of OLT.

The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.

Orthotopic의 쥐 모델 간 이식 Suprahepatic 베 나 정 맥 재건에 대 한 새로운 자석 문 합 기술을 사용 하 여

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model.

The goal of this study is to modify the rat orthotopic liver transplant model to better represent human liver transplantation and improve recipient survival. The presented method reestablishes hepatic arterial inflow by connecting the donor liver&#39;s common hepatic artery to the recipient liver&#39;s proper hepatic artery.

직교 간 이식의 쥐 모형에 있는 동맥 재연결 후에 감소된 합병증

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, including ischemia-reperfusion injury (IRI) of extrahepatic organs. This model requires numerous experiments and devices. The duration of anhepatic phase is closely related to the time to develop IRI after transplantation. In this experiment, we used hemodynamic changes to induce extrahepatic organ damage in rats and determined the maximum tolerance time. The time until the most severe organ injury varied for different organs. This method can easily be replicated and can also be used to study IRI of the extrahepatic organs after liver transplantation.

This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation.

쥐 간 이식 모형에 있는 혈역학적인 변경을 시뮬레이션하여 기관 허혈-재관류 상해

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

Strategies for efficient Vascular Anastomosis in Mouse Liver transplantation(마우스 간 이식에서 효율적인 혈관 문합을 위한 전략)

Vascular Reconstruction with the Cuff Technique in Mouse Orthotopic Liver Transplantation

Mouse orthotopic liver transplantation is an effective methodology for investigating the underlying mechanisms of liver ischemia and reperfusion injury. However, the technical challenges pose a barrier to utilizing this valuable experimental model and passing on these skills to the next generation. The most challenging aspect of this procedure is vascular reconstruction, including the portal vein (PV), infrahepatic inferior vena cava (IHIVC), and suprahepatic inferior vena cava. The use of plastic cuffs, rather than sutures, allows for smoother PV and IHIVC reconstruction. Vessels are reconstructed by attaching a cuff made from an intravenous catheter to the tip of the graft vessel and interposing the cuff into the recipient vessel. The two most crucial aspects are properly visualizing the inner lumen of the vessel and avoiding the use of excessive force. Our aim is to provide a technical overview of vascular reconstructions using the cuff technique in recipient surgery. These technical tips for the cuff technique are expected to help microsurgeons facilitate vascular reconstruction and advance their research.

저자 스포트라이트: 지방간 이식에서 한랭 허혈 및 온열 재관류 손상 탐구

This protocol provides technical information for vessel reconstruction using the cuff technique in mouse orthotopic liver transplantation.

Mouse Orthotopic Liver Transplantation에서 Cuff Technique을 사용한 혈관 재건

Mouse orthotopic liver transplantation is an effective methodology for investigating the underlying mechanisms of liver ischemia and reperfusion injury. ...

다시는 vivo에서 선박 지향 70% 쥐 부분 간 이식 Arterialized Hepatectomy

요약

더 많은 비디오 탐색

이 비디오의 챕터

쥐에게 Orthotopic 간 이식

간 간선 재건과 부분 Orthotopic 간 이식의 쥐 모델에 대한 외과 절차

이소성 보조 쥐 간 이식 급성 간 장애에 포털 정맥 Arterialization를 유량 조절

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일반 마우스의 70 % 부분 간 절제술 후 혈관 및 실질 재생의 시각화

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