The overall goal of this experiment is to identify and accurately quantitate over 10, 000 proteins from cell or tissue lysate across different drug treatments, time courses, or biological conditions. This method can help answer key questions in th
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We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.