February 5th, 2018
•This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.
Related Videos
Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
Live Imaging of Drosophila Larval Neuroblasts
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
Recording EEG in Freely Moving Neonatal Rats Using a Novel Method
Novel Assay for Cold Nociception in Drosophila Larvae
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유