Results: LD Index Measurements Enable the Study of TAG Dynamics in U. maydis
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Conclusion
필기록
The overall goal of this procedure is to determine the Lipid Droplet Index of cells cultured under different nutritional conditions. This method can help answer key questions in the Lipid Droplet and Lipid Field, such as the dynamic of accumulatio
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Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis.