Extraction of the Small Intestines from the Abdominal Cavity
3:11
Fabricating Intestinal Loops and Injecting them with Pathogens
4:58
Completing the Procedure and Collecting Results
6:44
Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
8:02
Conclusion
필기록
This method can help answer key questions in the field of Chicken Necrotic Enteritis research. Such as comparing the virulence of specific Clostridium perfringens strains causing this disease. The main advantage of this technique is that it remark
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Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.