Bulk Stabilization of Conventional hPSC Cultures for Subsequent Naïve Reversion with a Brief LIF-5i Adaptation
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Long-term Maintenance and Expansion of N-hPSC in LIF-3i Medium
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Results: Effective Reversion of Human Pluripotent Stem Cells
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Conclusion
필기록
The LIF-3i method reverts conventional human pluripotent stem cells into a more primitive pluripotent state with biochemical, transcriptional, and epigenetic features of the human pre-implantation epiblast. This method improves the functionality o
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We present a protocol for efficient, bulk, and rapid chemical reversion of conventional lineage-primed human pluripotent stem cells (hPSC) into an epigenomically-stable naïve preimplantation epiblast-like pluripotent state. This method results in decreased lineage-primed gene expression and marked improvement in directed multilineage differentiation across a broad repertoire of conventional hPSC lines.