Live Neutrophil Imaging and Neutrophil Extracellular Trap (NET) Detection and Quantification
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Results: Representative Canine Neutrophil Imaging and NET Detection
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Conclusion
필기록
This method can answer key questions about neutrophil extracellular trap or NET formation in dogs or other species. The main advantages of these techniques includes the real live detection of NET formation and identification of NET components like
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We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.