Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)
Manual Sorting of Fluorescently Labeled Mouse Neurons
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Results: Microdissection of GABAergic Neurons
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Conclusion
필기록
This method can help solve a key obstacle in the field of single-neuron isolation and sequencing. Such as collecting specific population or a subpopulation of fluorescently labeled neuron from user-defined brain area. The main advantage of this te
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This protocol describes the manual sorting procedure to isolate single fluorescently labeled neurons followed by in vitro transcription-based mRNA amplification for high-depth single-cell RNA sequencing.