January 9th, 2019
•This article illustrates a powerful method to quantify mitochondria or lysosomes in living cells. The combination of lysosome- or mitochondria-specific dyes with fluorescently conjugated antibodies against surface markers allows the quantification of these organelles in mixed cell populations, like primary cells harvested from tissue samples, by using multicolor flow cytometry.
Related Videos
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
Retroviral Transduction of T-cell Receptors in Mouse T-cells
piggyBac Transposon System Modification of Primary Human T Cells
The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
Copyright © 2024 MyJoVE Corporation. 판권 소유