November 21st, 2018
•Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.
Tags
Related Videos
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
Copyright © 2024 MyJoVE Corporation. 판권 소유