So the TBARS assay is best used for intra-laboratory general assessment of oxidative stress in biological samples, in which relative TBARS levels are directly compared between samples that were stored and processed together. These assay has an ext
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The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.