Results: Natural Transformation: Integration and Segregation of Insert, Vector for sfGFP, sfGFP Expression, and Motility Studies
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Conclusion
필기록
This protocol describes how filamentous cyanobacterium that belongs to oscillatoriales can be transformed by natural transformation. It also shows how different kinds of motion can be studied. Natural transformation is the simplest transformation
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Phormidium lacuna is a filamentous cyanobacterium that was isolated from marine rockpools. This article describes the isolation of filaments from natural sources, DNA extraction, genome sequencing, natural transformation, expression of sfGFP, cryoconservation, and motility methods.