Preparation of FD‐Glucose Solutions with Different Concentrations
4:19
Treating Starved 3T3‐L1 Cells
5:03
Extracellular and Intercellular Measurements for Stimulated 3T3‐L1 Cells
6:00
Protein‐Based Normalization of Intercellular Glucose Uptake
6:35
Ex Vivo Measurement of Extracellular FD‐Glucose Depletion in Organs
7:59
Results: Extracellular FD‐Glucose Correlation with Intracellular Uptake and Kinetics of Extracellular FD‐Glucose Depletion
9:39
Conclusion
필기록
Glucose uptake is the key to understanding health and disease. As glucose plays a pivotal role in metabolic and structural demands in the human body, as well as playing a vital role in regulation. The extracellular measurement of glucose allows fo
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Extracellular depletion of fluorescently labeled glucose correlates with glucose uptake and could be used for high-throughput screening of glucose uptake in excised organs and cell cultures.